A nearly 800 specimen collection of stoneflies from Crane Hollow Nature Preserve (CNHP) in Hocking County, Ohio was examined. These samples revealed a rich assemblage of 41 species, 40% of the species known from the state and 57% of the species known to occur in the lower Scioto River drainage. Species represented by the greatest abundance coincided well with those that were represented by the greatest number of sampling events. Of seven similar studies CHNP had substantially higher richness than all but Powdermill Preserve of southwestern Pennsylvania with 51 species. Assemblages varied greatly such that the average Jaccard dissimilarity between locations was 76%. Additional species are predicted for the CHNP. The value of aggregating literature and specimen data for comparative analyses is discussed as a way to place results of current biomonitoring and ecological studies into historical context.