Platelet-derived growth factor-BB (PDGF-BB) improves follicular survival, oocyte and follicular diameters, in a dose-dependent manner, after the in vitro culture of goat preantral follicles enclosed in ovarian tissue fragments

Renato Félix da Silva, Ivina Rocha Brito, Laritza Ferreira de Lima, Francisco Léo Nascimento de Aguiar, Giovanna Quintino Rodrigues, Ivila Lorrine Castro do Nascimento, Rebeca Magalhães Pedrosa Rocha, Francielli Weber Santos Cibin, Matthew Bryan Wheeler, Claudio Cabral Campello, Ana Paula Ribeiro Rodrigues, José Ricardo de Figueiredo

Research output: Contribution to journalArticlepeer-review

Abstract

The aims of this study were to investigate the effects of different concentrations of Platelet-derived growth factor-BB (PDGF-BB) on the survival, activation, levels of ROS, and growth of goat preantral follicles enclosed in ovarian tissue. For this, ovarian fragments were cultured for 7 days in Alpha Minimum Essential Medium (a-MEM+) with or without PDGF-BB (0, 25, 50 and 100 ng/ml). The results showed that both the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments maintained the percentage of morphologically normal follicles from day 1 to day 7. In addition, the 25 ng/ml PDGF treatment showed a significantly higher percentage of morphologically normal follicles when compared to the other treatments. At day 7, greater (P < 0.05) follicular and oocyte diameters were observed in the 25 ng/ml PDGF and the 50 ng/ml PDGF treatments when compared to the cultured control treatment. On day 7 of culture, all the treatments tested had a significant increase in the percentage of developing follicles when compared to the non-cultured control. However, the percentage of follicle activation, as well as ROS production, were similar (P < 0.05) among the treatments, irrespective of culture time. In conclusion, PDGF-BB improved, in a concentration-dependent manner, follicular survival as well as oocyte and follicular diameter after in vitro culture of goat preantral follicle-enclosed in ovarian tissue fragments.

Original languageEnglish (US)
Pages (from-to)1095-1102
Number of pages8
JournalAnimal Reproduction
Volume14
Issue number4
DOIs
StatePublished - 2017

Keywords

  • Goat
  • In vitro culture
  • PDGF-BB
  • Preantral follicles

ASJC Scopus subject areas

  • Animal Science and Zoology
  • General Veterinary

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