Plasmonic nanocone arrays for rapid and detailed cell lysate surface enhanced Raman spectroscopy analysis

L. P. Hackett, Lynford L Goddard, Gang Logan Liu

Research output: Contribution to journalArticle

Abstract

In this work, we develop, fabricate, and characterize a plasmonic nanocone array surface enhanced Raman spectroscopy (SERS) substrate with a uniform enhancement factor on the micron scale for qualitative and quantiative cell and cell lysate analysis. This work demonstrates how SERS substrates can be used as cell-based biosensors given that the enhancement factor of the substrate is sufficient for Raman detection and that the uniformity is high over the applicable surface area. These requirements allow accurate and quantitative comparisons between nonuniform samples under varying biochemical conditions. We apply the developed SERS substrate for Raman measurements and mapping of HeLa cells and cell lysate. This method is used for identification of UV-induced damage and detection of nanomolar concentrations of methylated guanine spiked in cell lysate samples.

Original languageEnglish (US)
Pages (from-to)4422-4430
Number of pages9
JournalAnalyst
Volume142
Issue number23
DOIs
StatePublished - Dec 7 2017

Fingerprint

Raman Spectrum Analysis
Raman spectroscopy
substrate
Substrates
Guanine
Biosensors
Biosensing Techniques
HeLa Cells
surface area
damage
analysis
detection

ASJC Scopus subject areas

  • Analytical Chemistry
  • Biochemistry
  • Environmental Chemistry
  • Spectroscopy
  • Electrochemistry

Cite this

Plasmonic nanocone arrays for rapid and detailed cell lysate surface enhanced Raman spectroscopy analysis. / Hackett, L. P.; Goddard, Lynford L; Liu, Gang Logan.

In: Analyst, Vol. 142, No. 23, 07.12.2017, p. 4422-4430.

Research output: Contribution to journalArticle

@article{7bfba8eefbe74a098fb5b4751326c7e4,
title = "Plasmonic nanocone arrays for rapid and detailed cell lysate surface enhanced Raman spectroscopy analysis",
abstract = "In this work, we develop, fabricate, and characterize a plasmonic nanocone array surface enhanced Raman spectroscopy (SERS) substrate with a uniform enhancement factor on the micron scale for qualitative and quantiative cell and cell lysate analysis. This work demonstrates how SERS substrates can be used as cell-based biosensors given that the enhancement factor of the substrate is sufficient for Raman detection and that the uniformity is high over the applicable surface area. These requirements allow accurate and quantitative comparisons between nonuniform samples under varying biochemical conditions. We apply the developed SERS substrate for Raman measurements and mapping of HeLa cells and cell lysate. This method is used for identification of UV-induced damage and detection of nanomolar concentrations of methylated guanine spiked in cell lysate samples.",
author = "Hackett, {L. P.} and Goddard, {Lynford L} and Liu, {Gang Logan}",
year = "2017",
month = "12",
day = "7",
doi = "10.1039/c7an00630f",
language = "English (US)",
volume = "142",
pages = "4422--4430",
journal = "Analyst",
issn = "0003-2654",
publisher = "Royal Society of Chemistry",
number = "23",

}

TY - JOUR

T1 - Plasmonic nanocone arrays for rapid and detailed cell lysate surface enhanced Raman spectroscopy analysis

AU - Hackett, L. P.

AU - Goddard, Lynford L

AU - Liu, Gang Logan

PY - 2017/12/7

Y1 - 2017/12/7

N2 - In this work, we develop, fabricate, and characterize a plasmonic nanocone array surface enhanced Raman spectroscopy (SERS) substrate with a uniform enhancement factor on the micron scale for qualitative and quantiative cell and cell lysate analysis. This work demonstrates how SERS substrates can be used as cell-based biosensors given that the enhancement factor of the substrate is sufficient for Raman detection and that the uniformity is high over the applicable surface area. These requirements allow accurate and quantitative comparisons between nonuniform samples under varying biochemical conditions. We apply the developed SERS substrate for Raman measurements and mapping of HeLa cells and cell lysate. This method is used for identification of UV-induced damage and detection of nanomolar concentrations of methylated guanine spiked in cell lysate samples.

AB - In this work, we develop, fabricate, and characterize a plasmonic nanocone array surface enhanced Raman spectroscopy (SERS) substrate with a uniform enhancement factor on the micron scale for qualitative and quantiative cell and cell lysate analysis. This work demonstrates how SERS substrates can be used as cell-based biosensors given that the enhancement factor of the substrate is sufficient for Raman detection and that the uniformity is high over the applicable surface area. These requirements allow accurate and quantitative comparisons between nonuniform samples under varying biochemical conditions. We apply the developed SERS substrate for Raman measurements and mapping of HeLa cells and cell lysate. This method is used for identification of UV-induced damage and detection of nanomolar concentrations of methylated guanine spiked in cell lysate samples.

UR - http://www.scopus.com/inward/record.url?scp=85034788156&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=85034788156&partnerID=8YFLogxK

U2 - 10.1039/c7an00630f

DO - 10.1039/c7an00630f

M3 - Article

C2 - 29068441

AN - SCOPUS:85034788156

VL - 142

SP - 4422

EP - 4430

JO - Analyst

JF - Analyst

SN - 0003-2654

IS - 23

ER -