Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmission

April M. Bobenchik, William H. Witola, Yoann Augagneur, Laura Nic Lochlainn, Aprajita Garg, Niseema Pachikara, Jae Yeon Choi, Yang O. Zhao, Sahar Usmani-Brown, Albert Lee, Sophie H. Adjalley, Swapna Samanta, David A. Fidock, Dennis R. Voelker, Erol Fikrig, Choukri Ben Mamoun

Research output: Contribution to journalArticle

Abstract

Efficient transmission of Plasmodium species between humans and Anopheles mosquitoes is a major contributor to the global burden of malaria. Gametocytogenesis, the process by which parasites switch from asexual replication within human erythrocytes to produce male and female gametocytes, is a critical step in malaria transmission and Plasmodium genetic diversity. Nothing is known about the pathways that regulate gametocytogenesis and only few of the current drugs that inhibit asexual replication are also capable of inhibiting gametocyte development and blocking malaria transmission. Here we provide genetic and pharmacological evidence indicating that the pathway for synthesis of phosphatidylcholine in Plasmodium falciparum membranes from host serine is essential for parasite gametocytogenesis and malaria transmission. Parasites lacking the phosphoethanolamine N-methyltransferase enzyme, which catalyzes the limiting step in this pathway, are severely altered in gametocyte development, are incapable of producing mature-stage gametocytes, and are not transmitted to mosquitoes. Chemical screening identified 11 inhibitors of phosphoethanolamine N-methyltransferase that block parasite intraerythrocytic asexual replication and gametocyte differentiation in the low micromolar range. Kinetic studies in vitro as well as functional complementation assays and lipid metabolic analyses in vivo on the most promising inhibitor NSC-158011 further demonstrated the specificity of inhibition. These studies set the stage for further optimization of NSC-158011 for development of a class of dual activity antimalarials to block both intraerythrocytic asexual replication and gametocytogenesis.

Original languageEnglish (US)
Pages (from-to)18262-18267
Number of pages6
JournalProceedings of the National Academy of Sciences of the United States of America
Volume110
Issue number45
DOIs
StatePublished - Nov 5 2013
Externally publishedYes

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Methyltransferases
Plasmodium falciparum
Malaria
Parasites
Culicidae
Plasmodium malariae
Anopheles
Plasmodium
Antimalarials
Phosphatidylcholines
Serine
Erythrocytes
Pharmacology
Lipids
Membranes
Enzymes
In Vitro Techniques

ASJC Scopus subject areas

  • General

Cite this

Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmission. / Bobenchik, April M.; Witola, William H.; Augagneur, Yoann; Lochlainn, Laura Nic; Garg, Aprajita; Pachikara, Niseema; Choi, Jae Yeon; Zhao, Yang O.; Usmani-Brown, Sahar; Lee, Albert; Adjalley, Sophie H.; Samanta, Swapna; Fidock, David A.; Voelker, Dennis R.; Fikrig, Erol; Mamoun, Choukri Ben.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 45, 05.11.2013, p. 18262-18267.

Research output: Contribution to journalArticle

Bobenchik, AM, Witola, WH, Augagneur, Y, Lochlainn, LN, Garg, A, Pachikara, N, Choi, JY, Zhao, YO, Usmani-Brown, S, Lee, A, Adjalley, SH, Samanta, S, Fidock, DA, Voelker, DR, Fikrig, E & Mamoun, CB 2013, 'Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmission' Proceedings of the National Academy of Sciences of the United States of America, vol 110, no. 45, pp. 18262-18267. DOI: 10.1073/pnas.1313965110

Bobenchik, April M.; Witola, William H.; Augagneur, Yoann; Lochlainn, Laura Nic; Garg, Aprajita; Pachikara, Niseema; Choi, Jae Yeon; Zhao, Yang O.; Usmani-Brown, Sahar; Lee, Albert; Adjalley, Sophie H.; Samanta, Swapna; Fidock, David A.; Voelker, Dennis R.; Fikrig, Erol; Mamoun, Choukri Ben / Plasmodium falciparum phosphoethanolamine methyltransferase is essential for malaria transmission.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 110, No. 45, 05.11.2013, p. 18262-18267.

Research output: Contribution to journalArticle

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