PLASMIDS IN CLOSTRIDIUM PERFRINGENS

MYRON SOLBERG, HANS P. BLASCHEK, PETER KAHN

Research output: Contribution to journalArticle

Abstract

A review of plasmids in Clostridium perfringens is presented. The characterization of the caseinase mediating plasmid pHB101 based upon limited DNase treatment, restriction endonuclease treatment, and agarose gel electrophoresis is described. Antibiotic profiles for the wild type strain and the cured strain were determined. The plasmid pHB101 was non self‐transferable. The curing procedure resulted in a stable morphological change from rod to coccoid or bacillary‐coccoid shape. A screening of C. perfringens strains showed the general presence of a 9.4 Mdal plasmid which may have been overlooked in the past due to chromosomal DNA masking effects.

Original languageEnglish (US)
Pages (from-to)267-289
Number of pages23
JournalJournal of Food Safety
Volume3
Issue number4
DOIs
StatePublished - Nov 1981

Fingerprint

Clostridium perfringens
plasmids
Plasmids
plasmid curing
deoxyribonucleases
Deoxyribonucleases
Agar Gel Electrophoresis
DNA Restriction Enzymes
restriction endonucleases
agarose
gel electrophoresis
antibiotics
Anti-Bacterial Agents
screening
DNA

ASJC Scopus subject areas

  • Parasitology
  • Food Science
  • Microbiology

Cite this

PLASMIDS IN CLOSTRIDIUM PERFRINGENS. / SOLBERG, MYRON; BLASCHEK, HANS P.; KAHN, PETER.

In: Journal of Food Safety, Vol. 3, No. 4, 11.1981, p. 267-289.

Research output: Contribution to journalArticle

SOLBERG, MYRON ; BLASCHEK, HANS P. ; KAHN, PETER. / PLASMIDS IN CLOSTRIDIUM PERFRINGENS. In: Journal of Food Safety. 1981 ; Vol. 3, No. 4. pp. 267-289.
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