TY - JOUR
T1 - Plasmid copy number and stability determination in Clostridium perfringens transformants
AU - Allen, Steven P.
AU - Blaschek, Hans P.
N1 - Funding Information:
This work was supported in part by University of Illinois Research Board grant 1-2-69647 and a University of Illinois Foundation Award to H.P.B. The authors would like to thank Antonius Suwanto for his technical help with rcgard to the operation of Beckman's TAFE system.
PY - 1990/11
Y1 - 1990/11
N2 - The copy number of the streptococcal plasmid pAMβ1 (26.5 kb), and its deletion derivatives, pVA1 (11 kb) and pVA677 (7.6 kb) contained in Clostridium perfringens 3624A transformants was determined by incorporation of [methyl-3-H]thymidine (4 μCi/ml) into chromosomal and plasmid DNA and sizing of the C. perfringens genome using transverse alternating field electrophoresis. Plasmids pAMβ1, pVA1, and pVA677 were found to be present at 1.0, 97, and 216 copies/cell, respectively, 10.2, 54, and 96% of the initial pAMβ1-, pVA1- and pVA677-containing transformants, respectively, remained resistant to erythromycin over 220 generations of growth. The results indicate a size-dependent relationship between plasmid stability and plasmid copy number in C. perfringens.
AB - The copy number of the streptococcal plasmid pAMβ1 (26.5 kb), and its deletion derivatives, pVA1 (11 kb) and pVA677 (7.6 kb) contained in Clostridium perfringens 3624A transformants was determined by incorporation of [methyl-3-H]thymidine (4 μCi/ml) into chromosomal and plasmid DNA and sizing of the C. perfringens genome using transverse alternating field electrophoresis. Plasmids pAMβ1, pVA1, and pVA677 were found to be present at 1.0, 97, and 216 copies/cell, respectively, 10.2, 54, and 96% of the initial pAMβ1-, pVA1- and pVA677-containing transformants, respectively, remained resistant to erythromycin over 220 generations of growth. The results indicate a size-dependent relationship between plasmid stability and plasmid copy number in C. perfringens.
KW - Genome size
KW - Plasmid copy number
KW - Plasmid stability
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U2 - 10.1016/0378-1097(90)90325-K
DO - 10.1016/0378-1097(90)90325-K
M3 - Article
C2 - 2083843
AN - SCOPUS:0025517914
SN - 0378-1097
VL - 72
SP - 323
EP - 327
JO - FEMS microbiology letters
JF - FEMS microbiology letters
IS - 3
ER -