TY - CHAP
T1 - Plant metabolomics
T2 - From experimental design to knowledge extraction
AU - Rai, Amit
AU - Umashankar, Shivshankar
AU - Swarup, Sanjay
PY - 2013
Y1 - 2013
N2 - Metabolomics is one of the most recent additions to the functional genomics approaches. It involves the use of analytical chemistry techniques to provide high-density data of metabolic profiles. Data is then analyzed using advanced statistics and databases to extract biological information, thus providing the metabolic phenotype of an organism. Large variety of metabolites produced by plants through the complex metabolic networks and their dynamic changes in response to various perturbations can be studied using metabolomics. Here, we describe the basic features of plant metabolic diversity and analytical methods to describe this diversity, which includes experimental workflows starting from experimental design, sample preparation, hardware and software choices, combined with knowledge extraction methods. Finally, we describe a scenario for using these workflows to identify differential metabolites and their pathways from complex biological samples.
AB - Metabolomics is one of the most recent additions to the functional genomics approaches. It involves the use of analytical chemistry techniques to provide high-density data of metabolic profiles. Data is then analyzed using advanced statistics and databases to extract biological information, thus providing the metabolic phenotype of an organism. Large variety of metabolites produced by plants through the complex metabolic networks and their dynamic changes in response to various perturbations can be studied using metabolomics. Here, we describe the basic features of plant metabolic diversity and analytical methods to describe this diversity, which includes experimental workflows starting from experimental design, sample preparation, hardware and software choices, combined with knowledge extraction methods. Finally, we describe a scenario for using these workflows to identify differential metabolites and their pathways from complex biological samples.
KW - Arabidopsis
KW - Extraction
KW - Fragmentation of metabolites
KW - Homogenization
KW - LCMS
KW - Metabolic profiling
KW - Metabolite identification
KW - Metabolomics data mining
UR - http://www.scopus.com/inward/record.url?scp=84884179262&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84884179262&partnerID=8YFLogxK
U2 - 10.1007/978-1-62703-613-9_19
DO - 10.1007/978-1-62703-613-9_19
M3 - Chapter
C2 - 23996322
AN - SCOPUS:84884179262
SN - 9781627036122
T3 - Methods in Molecular Biology
SP - 279
EP - 312
BT - Legume Genomics
A2 - Rose, Ray
ER -