Plant intron sequences: Evidence for distinct groups of introns

Brian A. Hanley, Mary A. Schuler

Research output: Contribution to journalArticle

Abstract

In vivo and in vitro RNA splicing experiments have demonstrated that the intron splicing machineries are not interchangeable in all organisms. These differences have prevented the efficient in vivo expression of monocot genes containing introns in dicot plants and the in vitro excision of some plant introns in HeLa cell in vitro splicing extracts. We have analyzed plant introns for sequence differences which potentially account for the functional splicing differences. Three classes of plant introns can be differentiated by the purine or pyrimidine-richness of sequences upstream from the 3′ splice site. The frequency of these three types of introns in monocots and dicots varies significantly. The degree of variability in the 5′ and 31 intron boundaries is evaluated for each of these classes in monocots and dicots. The 5′ splice site consensus sequences developed for the monocot and dicot introns differ in their ability to base pair with conserved nucleotides present at the 5′ end of many U1 snRNAs.

Original languageEnglish (US)
Pages (from-to)7159-7176
Number of pages18
JournalNucleic acids research
Volume16
Issue number14
DOIs
StatePublished - Jul 25 1988

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Introns
RNA Splice Sites
RNA Splicing
Consensus Sequence
HeLa Cells
Base Pairing
Nucleotides
Gene Expression
In Vitro Techniques

ASJC Scopus subject areas

  • Genetics

Cite this

Plant intron sequences : Evidence for distinct groups of introns. / Hanley, Brian A.; Schuler, Mary A.

In: Nucleic acids research, Vol. 16, No. 14, 25.07.1988, p. 7159-7176.

Research output: Contribution to journalArticle

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