Plant extracts from Central Asia showing antiinflammatory activities in gene expression assays

Moul Dey, Christophe Ripoll, Reneta Pouleva, Ruth Dorn, Irina Aranovich, David Zaurov, Anvar Kurmukov, Marietta Eliseyeva, Igor Belolipov, Anarbek Akimaliev, Ishenbay Sodombekov, Djamin Akimaliev, Mary Ann Lila, Ilya Raskin

Research output: Contribution to journalArticlepeer-review

Abstract

Plant natural products remain a good resource for the discovery of novel pharmaceuticals. A mouse macrophage-based quantitative, reverse transcription polymerase chain reaction (qRT-PCR) system was optimized to screen plant extracts for antiinflammatory activities using three well known genetic markers of inflammation. Plants used for extraction were taxonomically identified and vouchered species from two Central Asian countries, Uzbekistan and Kyrgyzstan, collected through the International Cooperative Biodiversity Groups (ICBG) program. The mRNA expression of cyclooxygenase-2, interleukin 1β and inducible nitric oxide synthase genes in RAW macrophages was determined quantitatively in response to treatment with plant extracts applied at 100 μg/mL. The screening of 1000 extracts from 449 plant species belonging to 68 plant families resulted in 75 extracts (7.5%) showing strong (75% or higher inhibition) activity against at least one target gene. Many extracts showed qualitative and quantitative differences in the levels of activities against each target gene. Extracts identified from this screen were able to reduce inflammatory symptoms in vivo, thereby validating the screening approach.

Original languageEnglish (US)
Pages (from-to)929-934
Number of pages6
JournalPhytotherapy Research
Volume22
Issue number7
DOIs
StatePublished - Jul 2008

Keywords

  • Antiinflammatory natural product
  • Gene expression
  • Real-time RT-PCR

ASJC Scopus subject areas

  • Pharmacology

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