Plant cells at different stages in their growth curve differentially activate promutagens

James M. Gentile, Deborah Heydenburg, Lori Hertel, Michael Jacob Plewa

Research output: Contribution to journalArticle

Abstract

Mutagenic activity of the promutagens 2-aminofluorene (2AF) and a contaminant of 4-nitro-o-phenylenediamine (NOP-X) was followed in Ames Salmonella strain TA98 following metabolism by cotton and carrot cell suspension cultures using the plant cell/microbe coincubation assay. Both cell lines were capable of activating each chemical. However, activation capacities of the cell lines differed relative to their respective stage of growth when used. For 2AF activation early-log phase cotton cells and mid-log phase carrot cells proved superior while mid-log phase cotton cells and stationary phase carrot cells proved superior for NOP-X activation. These data indicate that the phase of the growth cycle at which plant cells are harvested can significantly affect their promutagen activation potential.

Original languageEnglish (US)
Pages (from-to)151-155
Number of pages5
JournalMutation Research Letters
Volume191
Issue number3-4
DOIs
StatePublished - Jan 1 1987

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Plant Cells
Daucus carota
Growth
Cell Line
Salmonella
Suspensions
Cell Culture Techniques
2-aminofluorene

Keywords

  • 2-Aminofluorene
  • 4-Nitro-o-phenylenediamine
  • Plant cells
  • Promutagen activation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Plant cells at different stages in their growth curve differentially activate promutagens. / Gentile, James M.; Heydenburg, Deborah; Hertel, Lori; Plewa, Michael Jacob.

In: Mutation Research Letters, Vol. 191, No. 3-4, 01.01.1987, p. 151-155.

Research output: Contribution to journalArticle

Gentile, James M. ; Heydenburg, Deborah ; Hertel, Lori ; Plewa, Michael Jacob. / Plant cells at different stages in their growth curve differentially activate promutagens. In: Mutation Research Letters. 1987 ; Vol. 191, No. 3-4. pp. 151-155.
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