Abstract
Benzidine and 4-aminobiphenyl (4-ABP) are promutagenic bicyclic aromatic amines that are activated into frameshift and base pair substitution mutagens by plant systems. Using the plant cell/microbe coincubation assay, plant-activated benzidine from 0 to 50 μM induced a concentration-response in Salmonella typhimurium. At concentrations above 5 μM, plant-activated benzidine induced frameshift and base pair substitution mutations in the N- or O-acetyltransferase over-expressing strains, DJ460, YG1024, and YG1029. With plant-activated 4-ABP, concentrations above 250 μM induced a significant mutagenic response in strains YG1024 and YG1029. A tobacco cell-free mixture, TX1MX, activated benzidne and 4-ABP into mutagenic metabolites in S. typhimurium strains YG1024, YG1029, and DJ460. The mutagenic sensitivities of plant-activated benzidine and 4-ABP were the same with two different types of plant activation systems, TX1 suspension cells and TX1MX cell-free medium. The plant activation of these aromatic amines is mediated by tobacco cell peroxidase. Plant-activated benzidine and 4-ABP are converted into intermediates that serve as substrates for bacterial or humanacetylCoA: N-hydroxyarylamine N-acetyltransferase to generate the ultimate mutagenic products.
Original language | English (US) |
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Pages (from-to) | 81-90 |
Number of pages | 10 |
Journal | Environmental and Molecular Mutagenesis |
Volume | 29 |
Issue number | 1 |
DOIs | |
State | Published - Feb 24 1997 |
Keywords
- 4-Aminobiphenyl
- Benzidine
- Carbon monoxide
- Diethyldithiocarbamate
- O-acetyltransferase
- Plant activation
- Salmonella typhimurium
- TX1
- TX1MX
ASJC Scopus subject areas
- Epidemiology
- Genetics(clinical)
- Health, Toxicology and Mutagenesis