Pituitary adenylate cyclase activating peptide (PACAP) in the retinohypothalamic tract: A daytime regulator of the biological clock

Jens Hannibal, Jian M. Ding, Dong Chen, Jan Fahrenkrug, Philip J. Larsen, Martha U. Gillette, Jens D. Mikkelsen

Research output: Contribution to journalArticle

Abstract

The retinohypothalamic tract (RHT) relays photic information from the eyes to the brain biological clock in the suprachiasmatic nucleus (SCN). Activation of this pathway by light plays a role in adjusting circadian timing to light exposure at night. Here we report a new signaling pathway by which the RHT regulates circadian timing in the daytime as well. Using dual-immunocytochemistry for PACAP and the in vivo tracer Cholera toxin subunit B (ChB), intense PACAP immunoreactivity (PACAP-IR) was observed in retinal afferents at the rat SCN as web as in the intergeniculate leaflet (IGL) of the thalamus. This PACAP-IR was nearly lost upon bilateral eye enucleation. PACAP afferents originated from ganglion cells distributed throughout the retina. The phase of circadian rhythm measured as SCN neuronal activity in vitro was significantly advanced by application of PACAP-38 during the subjective day, but not at night. The effect is channelled to the clock via a PACAP 1 receptor-cAMP signaling mechanism. Thus, in addition to its role in nocturnal regulation by glutamatergic neurotransmission, the RHT can adjust the biological clock by a PACAP cAMP-dependent mechanism during the daytime.

Original languageEnglish (US)
Pages (from-to)197-206
Number of pages10
JournalAnnals of the New York Academy of Sciences
Volume865
DOIs
StatePublished - Jan 1 1998

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Biological Clocks
Adenylyl Cyclases
Clocks
Peptides
Suprachiasmatic Nucleus
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Eye Enucleation
Light
Cyclic AMP Receptors
Cholera Toxin
Circadian Rhythm
Thalamus
Synaptic Transmission
Ganglia
Retina
Nucleus
Clock
Immunohistochemistry
Rats
Brain

ASJC Scopus subject areas

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Pituitary adenylate cyclase activating peptide (PACAP) in the retinohypothalamic tract : A daytime regulator of the biological clock. / Hannibal, Jens; Ding, Jian M.; Chen, Dong; Fahrenkrug, Jan; Larsen, Philip J.; Gillette, Martha U.; Mikkelsen, Jens D.

In: Annals of the New York Academy of Sciences, Vol. 865, 01.01.1998, p. 197-206.

Research output: Contribution to journalArticle

Hannibal, Jens ; Ding, Jian M. ; Chen, Dong ; Fahrenkrug, Jan ; Larsen, Philip J. ; Gillette, Martha U. ; Mikkelsen, Jens D. / Pituitary adenylate cyclase activating peptide (PACAP) in the retinohypothalamic tract : A daytime regulator of the biological clock. In: Annals of the New York Academy of Sciences. 1998 ; Vol. 865. pp. 197-206.
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