TY - JOUR
T1 - Piscinibacter caeni sp. Nov., isolated from activated sludge
AU - Chen, Dong Zhi
AU - Yu, Ning Ning
AU - Chu, Qi Ying
AU - Chen, Jing
AU - Ye, Jie Xu
AU - Cheng, Zhuo Wei
AU - Zhang, Shi Han
AU - Chen, Jian Meng
N1 - This work was financially supported by the National Natural Science Foundation of China (Nos. 21477116 and 51778581) and the Natural Science Foundation of Zhejiang Province (LY17E080019).
PY - 2018/8
Y1 - 2018/8
N2 - A yellowish-pigmented bacterial strain, designated as MQ-18T, was isolated from a sample of activated sludge collected from a pharmaceutical factory in Zhejiang, China. The strain was characterized through a polyphasic taxonomy approach. 16S rRNA gene sequence analysis demonstrated that strain MQ-18T showed high similarities to Piscinibacter defluvii SH-1T (99.7 %) and Piscinibacter aquaticus IMCC1728T (98.4 %), thereby suggesting that it belongs to the genus Piscinibacter. The DNA–DNA relatedness values of this strain to strains SH-1T and IMCC1728T were only 35.4 and 33.3 %, respectively. Cells of MQ-18T were Gram-negative, aerobic, motile, rod-shaped and non-spore forming. This strain exhibited growth at 25–37° C (optimum:30° C) in the presence of 0–3.0 % (w/v) NaCl (optimum, 0 % NaCl) and at pH 5.0–8.0 (pH 7.0). The predominant fatty acids were C12:0 (5.5 %), C16:0 (33.7 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 38.5 %), and summed feature 4 (anteiso-C17:1 B and/or iso C17:1 I; 11.6 %). The main quinone type was ubiquinone-8, and the major polyamines were cadaverine and putrescine. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 70.1 mol%. On the basis of its phylogenetic, phenotypic and physiological characteristics, strain MQ-18T is considered to represent a novel species of the genus Piscinibacter, for which the name Piscinibacter caeni sp. nov. is proposed. The type strain is MQ-18T (CCTCC AB 2017223T =JCM 32138T).
AB - A yellowish-pigmented bacterial strain, designated as MQ-18T, was isolated from a sample of activated sludge collected from a pharmaceutical factory in Zhejiang, China. The strain was characterized through a polyphasic taxonomy approach. 16S rRNA gene sequence analysis demonstrated that strain MQ-18T showed high similarities to Piscinibacter defluvii SH-1T (99.7 %) and Piscinibacter aquaticus IMCC1728T (98.4 %), thereby suggesting that it belongs to the genus Piscinibacter. The DNA–DNA relatedness values of this strain to strains SH-1T and IMCC1728T were only 35.4 and 33.3 %, respectively. Cells of MQ-18T were Gram-negative, aerobic, motile, rod-shaped and non-spore forming. This strain exhibited growth at 25–37° C (optimum:30° C) in the presence of 0–3.0 % (w/v) NaCl (optimum, 0 % NaCl) and at pH 5.0–8.0 (pH 7.0). The predominant fatty acids were C12:0 (5.5 %), C16:0 (33.7 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 38.5 %), and summed feature 4 (anteiso-C17:1 B and/or iso C17:1 I; 11.6 %). The main quinone type was ubiquinone-8, and the major polyamines were cadaverine and putrescine. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 70.1 mol%. On the basis of its phylogenetic, phenotypic and physiological characteristics, strain MQ-18T is considered to represent a novel species of the genus Piscinibacter, for which the name Piscinibacter caeni sp. nov. is proposed. The type strain is MQ-18T (CCTCC AB 2017223T =JCM 32138T).
KW - Activated sludge
KW - New species
KW - Piscinibacter caeni
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U2 - 10.1099/ijsem.0.002891
DO - 10.1099/ijsem.0.002891
M3 - Article
C2 - 29957173
AN - SCOPUS:85051213188
SN - 1466-5026
VL - 68
SP - 2627
EP - 2632
JO - International Journal of Systematic and Evolutionary Microbiology
JF - International Journal of Systematic and Evolutionary Microbiology
IS - 8
M1 - 002891
ER -