Piscinibacter caeni sp. Nov., isolated from activated sludge

Dong Zhi Chen, Ning Ning Yu, Qi Ying Chu, Jing Chen, Jie Xu Ye, Zhuo Wei Cheng, Shi Han Zhang, Jian Meng Chen

Research output: Contribution to journalArticlepeer-review

Abstract

A yellowish-pigmented bacterial strain, designated as MQ-18T, was isolated from a sample of activated sludge collected from a pharmaceutical factory in Zhejiang, China. The strain was characterized through a polyphasic taxonomy approach. 16S rRNA gene sequence analysis demonstrated that strain MQ-18T showed high similarities to Piscinibacter defluvii SH-1T (99.7 %) and Piscinibacter aquaticus IMCC1728T (98.4 %), thereby suggesting that it belongs to the genus Piscinibacter. The DNA–DNA relatedness values of this strain to strains SH-1T and IMCC1728T were only 35.4 and 33.3 %, respectively. Cells of MQ-18T were Gram-negative, aerobic, motile, rod-shaped and non-spore forming. This strain exhibited growth at 25–37° C (optimum:30° C) in the presence of 0–3.0 % (w/v) NaCl (optimum, 0 % NaCl) and at pH 5.0–8.0 (pH 7.0). The predominant fatty acids were C12:0 (5.5 %), C16:0 (33.7 %), summed feature 3 (C16:1ω7c and/or C16:1ω6c; 38.5 %), and summed feature 4 (anteiso-C17:1 B and/or iso C17:1 I; 11.6 %). The main quinone type was ubiquinone-8, and the major polyamines were cadaverine and putrescine. The major polar lipid profile consisted of diphosphatidylglycerol, phosphatidylglycerol and phosphatidylethanolamine. The DNA G+C content was 70.1 mol%. On the basis of its phylogenetic, phenotypic and physiological characteristics, strain MQ-18T is considered to represent a novel species of the genus Piscinibacter, for which the name Piscinibacter caeni sp. nov. is proposed. The type strain is MQ-18T (CCTCC AB 2017223T =JCM 32138T).

Original languageEnglish (US)
Article number002891
Pages (from-to)2627-2632
Number of pages6
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume68
Issue number8
DOIs
StatePublished - Aug 2018

Keywords

  • Activated sludge
  • New species
  • Piscinibacter caeni

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

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