Pilot-scale production of butanol by Clostridium beijerinckii BA101 using a low-cost fermentation medium based on corn steep water

M. Parekh, J. Formanek, H. P. Blaschek

Research output: Contribution to journalArticlepeer-review

Abstract

To improve the economic competitiveness of the acetone/butanol/ethanol fermentation process, glucose/corn steep water (CSW) medium was used on a pilot scale for the production of solvents. The production of butanol by the Clostridium beijerinckii NCIMB 8052 parent strain and the solvent- hyperproducing BA101 mutant was compared. In a 20-1 fermentation using 5% glucose/CSW medium, C. beijerinckii 8052 produced 8.5 g butanol/l and 5 g acetone/l, while C. beijerinckii BA101 produced 16 g butanol/l and 7.5 g acetone/l. Further studies were carried out on a larger scale using an optimized 6% glucose/CSW medium. In a 200-1 pilot-scale fermentor, C. beijerinckii 8052 produced 12.7 g butanol/l and 6 g acetone/l following 96 h of fermentation. C. beijerinckii BA101 produced 17.8 g/l and 5.5 g/l butanol and acetone respectively, following 130 h of fermentation. These results represent a 40% increase in final butanol concentration by the C. beijerinckii BA101 mutant strain when compared to the 8052 parent strain. The total solvents (acetone, butanol, and ethanol) produced by C. beijerinckii NCIMB 8052 and BA101 in a 200-l fermentation were 19.2 g/l and 23.6 g/l respectively. This is the first report of pilot-scale butanol production by the solvent-hyperproducing C. beijerinckii BA101 mutant employing an inexpensive glucose/CSW medium.

Original languageEnglish (US)
Pages (from-to)152-157
Number of pages6
JournalApplied Microbiology and Biotechnology
Volume51
Issue number2
DOIs
StatePublished - Mar 13 1999

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

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