Pig BVDV-2 non-structural protein (Npro) links to cellular antiviral response in vitro

Jie Tao, Jinhu Liao, Jianye Wang, Xinjun Zhang, Qian Zhang, Liqian Zhu, Weiping Zhang, Huili Liu, Guoqiang Zhu

Research output: Contribution to journalArticlepeer-review

Abstract

In this study, we constructed for the first time a full-length cDNA clone of pig-original bovine viral diarrhea virus 2 (BVDV-2) strain SH-28, modified the cDNA clone (pASH28) for mutant pASHΔNpro and derived virus strain vASHΔNpro by deleting the genomic region encoding the Npro polypeptide, and examined significance of protein Npro for antiviral responses in vitro. Data showed that Npro-deletion mutant virus vASHΔNpro led to significant overexpression of oligo adenylate synthetase (OAS), myxovirus-resistant protein 1 (Mx1), and ubiquitin-like protein 15 (ISG15). Data also revealed that overexpression of Npro, but not NS2 and NS3 proteins, resulted in significant down-regulation of OAS, Mx1, and ISG15 production (p ≤ 0.05) in bovine cells as well as porcine cells transfected with Npro recombinant eukaryotic expression plasmids. Npro (but not NS2 and NS3) was also found to inhibit poly(IC) from inducing production of type I interferon (IFN-I). These results indicated that protein Npro may play multiple roles in regulating antiviral response in host cells interfered by pig BVDV-2 strain, and provided useful information to understand better the mechanism of BVDV-2 persistent infection in pigs.

Original languageEnglish (US)
Pages (from-to)233-239
Number of pages7
JournalVirus Genes
Volume53
Issue number2
DOIs
StatePublished - Apr 1 2017
Externally publishedYes

Keywords

  • Antiviral response
  • BVDV-2
  • Infectious cDNA clone
  • Non-structural protein
  • Pig

ASJC Scopus subject areas

  • Molecular Biology
  • Genetics
  • Virology

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