Phytoecdysteroids increase protein synthesis in skeletal muscle cells

Jonathan Gorelick-Feldman; David MacLean; Nebojsa Ilic; Alexander Poulev; Mary Ann Lila; Diana Cheng; Ilya Raskin

doi:10.1021/jf073059z

Phytoecdysteroids increase protein synthesis in skeletal muscle cells

Jonathan Gorelick-Feldman, David MacLean, Nebojsa Ilic, Alexander Poulev, Mary Ann Lila, Diana Cheng, Ilya Raskin

Natural Resources and Environmental Sciences

Research output: Contribution to journal › Article › peer-review

Abstract

Phytoecdysteroids, which are structurally similar or identical to insect molting hormones, produce a range of effects in mammals, including increasing growth and physical performance. To study the mechanism of action of phytoecdysteroids in mammalian tissue, an in vitro cellular assay of protein synthesis was developed. In C2C12 murine myotubes and human primary myotubes, phytoecdy steroids increased protein synthesis by up to 20%. In vivo, ecdysteroids increased rat grip strength. Ecdysteroid-containing plant extracts produced similar results. The effect was inhibited by a phosphoinositide kinase-3 inhibitor, which suggests a PI3K-mediated mechanism.

Original language	English (US)
Pages (from-to)	3532-3537
Number of pages	6
Journal	Journal of Agricultural and Food Chemistry
Volume	56
Issue number	10
DOIs	https://doi.org/10.1021/jf073059z
State	Published - May 28 2008

Keywords

Ajuga
C2C12
Ecdysteroid
Myotube
Protein synthesis
Skeletal muscle
Spinach

ASJC Scopus subject areas

General Chemistry
General Agricultural and Biological Sciences

Online availability

10.1021/jf073059z

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abstract = "Phytoecdysteroids, which are structurally similar or identical to insect molting hormones, produce a range of effects in mammals, including increasing growth and physical performance. To study the mechanism of action of phytoecdysteroids in mammalian tissue, an in vitro cellular assay of protein synthesis was developed. In C2C12 murine myotubes and human primary myotubes, phytoecdy steroids increased protein synthesis by up to 20%. In vivo, ecdysteroids increased rat grip strength. Ecdysteroid-containing plant extracts produced similar results. The effect was inhibited by a phosphoinositide kinase-3 inhibitor, which suggests a PI3K-mediated mechanism.",

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AU - Gorelick-Feldman, Jonathan

AU - MacLean, David

AU - Ilic, Nebojsa

AU - Poulev, Alexander

AU - Lila, Mary Ann

AU - Cheng, Diana

AU - Raskin, Ilya

PY - 2008/5/28

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Phytoecdysteroids increase protein synthesis in skeletal muscle cells

Abstract

Keywords

ASJC Scopus subject areas

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