Phytoecdysteroids do not have anabolic effects in skeletal muscle in sedentary aging mice

Marcus M. Lawrence, Kevin A. Zwetsloot, Susan T. Arthur, Chase A. Sherman, Joshua R. Huot, Vladimir Badmaev, Mary Grace, Mary Ann Lila, David C. Nieman, R. Andrew Shanely

Research output: Contribution to journalArticlepeer-review


Skeletal muscle mass and strength are lost with aging. Phytoecdysteroids, in particular 20-hydroxyecdysone (20E), increase protein synthesis in C2C12 skeletal muscle cells and muscle strength in young rats. The objective of this study was to determine whether an extract from Ajuga turkestanica (ATE), enriched in phytoecdysteroids, and 20E affect skeletal muscle mass and fiber size, fiber type, activation of the PI3K–Akt signaling pathway, and the mRNA levels of MAFbx, MuRF-1, and myostatin in sedentary aging mice. Aging male C57BL/6 mice (20 months old) received ATE, 20E, or vehicle (CT) once per day for 28 days or a single acute dose. Treatment did not alter body, muscle, or organ mass; fiber cross-sectional area; or fiber type in the triceps brachii or plantaris muscles. Likewise, protein synthesis signaling markers (i.e., phosphorylation of AktSer473 and p70S6kThr389) measured after either 28 days or acutely were unchanged. Neither ATE nor 20E treatment for 28 days affected the mRNA levels of MAFbx, MuRF-1, and myostatin. In conclusion, these data indicate that phytoecdysteroid treatment does not alter muscle mass or fiber type, nor does it activate protein synthesis signaling in the skeletal muscle of sedentary aging mice.

Original languageEnglish (US)
Article number370
Pages (from-to)1-14
Number of pages14
JournalInternational journal of environmental research and public health
Issue number2
StatePublished - Jan 2 2021
Externally publishedYes


  • 20-hydroxyecdysone
  • Akt
  • Atrogenes
  • Hypertrophy
  • P70S6K
  • Skeletal muscle

ASJC Scopus subject areas

  • Pollution
  • Public Health, Environmental and Occupational Health
  • Health, Toxicology and Mutagenesis


Dive into the research topics of 'Phytoecdysteroids do not have anabolic effects in skeletal muscle in sedentary aging mice'. Together they form a unique fingerprint.

Cite this