Phytic Acid Determination in Soybeans

D. B. THOMPSON, J. W. ERDMAN

Research output: Contribution to journalArticle

Abstract

Several established methods of phytic acid determination in soybeans were evaluated. Iron analysis methods, which rely on a 4:6 molar ratio of Fe:P, were eliminated because this ratio was not dependable. Three assay methods relying on phosphorus analysis were then compared. The anion‐exchange method was considered most accurate but not convenient for routine analysis. Analysis of the ferric phytate precipitate and a new method, analysis of the supernatant before and after ferric chloride precipitation, were judged against the anion‐exchange method under different extraction conditions. Based upon good agreement with anion‐exchange column results and acceptable reproducibility, the best methods were (1) precipitate analysis of phosphorus after extraction with 3% TCA + 10% sodium sulfate, or (2) the supernatant difference method after extraction with 1.2% HCl.

Original languageEnglish (US)
Pages (from-to)513-517
Number of pages5
JournalJournal of food science
Volume47
Issue number2
DOIs
StatePublished - Mar 1982

ASJC Scopus subject areas

  • Food Science

Fingerprint Dive into the research topics of 'Phytic Acid Determination in Soybeans'. Together they form a unique fingerprint.

  • Cite this