@article{19204945e6944d1a89032718b93cdb1c,
title = "Physiological stress drives the emergence of a Salmonella subpopulation through ribosomal RNA regulation",
abstract = "Bacteria undergo cycles of growth and starvation to which they must adapt swiftly. One important strategy for adjusting growth rates relies on ribosomal levels. Although high ribosomal levels are required for fast growth, their dynamics during starvation remain unclear. Here, we analyzed ribosomal RNA (rRNA) content of individual Salmonella cells by using fluorescence in situ hybridization (rRNA-FISH) and measured a dramatic decrease in rRNA numbers only in a subpopulation during nutrient limitation, resulting in a bimodal distribution of cells with high and low rRNA content. During nutritional upshifts, the two subpopulations were associated with distinct phenotypes. Using a transposon screen coupled with rRNA-FISH, we identified two mutants, DksA and RNase I, acting on rRNA transcription shutdown and degradation, which abolished the formation of the subpopulation with low rRNA content. Our work identifies a bacterial mechanism for regulation of ribosomal bimodality that may be beneficial for population survival during starvation.",
keywords = "Salmonella, ribosomal RNA, single cell, smFISH, stress response",
author = "{Ciolli Mattioli}, Camilla and Kfir Eisner and Aviel Rosenbaum and Mengyu Wang and Andre' Rivalta and Ariel Amir and Ido Golding and Roi Avraham",
note = "This research was supported by the European Research Committee (ERC grant No. 756653), the Israel Science Foundation (ISF grant No. 1289/22), and the Minerva Foundation with funding from the Federal Ministry for Education and Research. C.C.M. received funding from the European Union's Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant No. 898715. Work in the Golding lab is supported by the National Institutes of Health grant R35 GM140709 and by the Alfred P. Sloan Foundation. We acknowledge Prof. Ada Yonath's lab for discussions and help with ribosome analysis and the Smoler Proteomics Center at the Technion for performing the mass spectrometry analysis. Conceptualization and methodology, C.C.M. and R.A.; investigation, C.C.M. performed the experiments, A. Rosenbaum performed bulk upshift experiments, A. Rivalta assisted in the sucrose cushion experiment; formal analysis, C.C.M. and K.E.; writing – original draft, C.C.M. and K.E.; writing – review & editing, C.C.M. K.E. R.A. I.G. and A.A.; funding acquisition, C.C.M. and R.A. The authors declare no competing interests. We support inclusive, diverse, and equitable conduct of research. This research was supported by the European Research Committee (ERC grant No. 756653 ), the Israel Science Foundation (ISF grant No. 1289/22 ), and the Minerva Foundation with funding from the Federal Ministry for Education and Research . C.C.M. received funding from the European Union{\textquoteright}s Horizon 2020 research and innovation programme under the Marie Sk{\l}odowska-Curie grant No. 898715 . Work in the Golding lab is supported by the National Institutes of Health grant R35 GM140709 and by the Alfred P. Sloan Foundation . We acknowledge Prof. Ada Yonath{\textquoteright}s lab for discussions and help with ribosome analysis and the Smoler Proteomics Center at the Technion for performing the mass spectrometry analysis.",
year = "2023",
month = nov,
day = "20",
doi = "10.1016/j.cub.2023.09.064",
language = "English (US)",
volume = "33",
pages = "4880--4892",
journal = "Current Biology",
issn = "0960-9822",
publisher = "Cell Press",
number = "22",
}