Physiological and chemical inducers of tissue factor pathway inhibitor-2 in human glioma cells.

Santhi D. Konduri, Niranjan Yanamandra, Dzung H. Dinh, William C. Olivero, Meena Gujrati, Donald C. Foster, Walter Kisiel, Jasti S. Rao

Research output: Contribution to journalArticle

Abstract

Tissue factor pathway inhibitor-2 (TFPI-2), a serine protease inhibitor abundant in the extracellular matrix, is expressed in high amounts in low-grade, non-invasive glioma cells but in low amounts in high-grade, highly invasive glioma cells. Overexpression of TFPI-2 by highly invasive glioma cells reduces their invasiveness and thus may be useful in cancer therapy. The mechanisms underlying the transcriptional regulation of TFPI-2 are not well elucidated. We previously reported that the -312 to +1 region of TFPI-2 was critical for the minimal, inducible regulation of TFPI-2 in gliomas. This region harbors sites for several transcription factors, including SP1 (-192 to -183 and -135 to -128), AP-1 (-310 to -300, -213 to -204, and -163 to -154), NF-kappaB (-229 to -221), an NF-kappaB-like site (-291 to -281), and Lyf-1 (-260 to -252). Here we transiently transfected low-grade Hs683 glioma cells with mutant constructs to clarify the role of these transcription factors in TFPI-2 regulation. Addition of phorbol 12-myristate 13-acetate, 1,2-diacyl-sn-glycerol, IFN-gamma, or IFN-alpha induced the expression of TFPI-2 wild-type promoter construct as well as TFPI-2 protein and mRNA in Hs683 cells. Mutations at either of two AP-1 sites (-310 to -300 and -163 to -154) or either of two SP1 sites (-192 to -183 and -135 to -128) resulted in reduced TFPI-2 activity, regardless of the presence of stimulator compounds, and reduction in DNA-protein binding (by electrophoretic mobility shift assay).

Original languageEnglish (US)
Pages (from-to)1277-1283
Number of pages7
JournalInternational journal of oncology
Volume22
Issue number6
StatePublished - Jan 1 2003

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Glioma
NF-kappa B
Transcription Factor AP-1
Transcription Factors
tissue-factor-pathway inhibitor 2
Serine Proteinase Inhibitors
DNA-Binding Proteins
Electrophoretic Mobility Shift Assay
Glycerol
Extracellular Matrix
Acetates
Messenger RNA
Mutation

ASJC Scopus subject areas

  • Oncology
  • Cancer Research

Cite this

Konduri, S. D., Yanamandra, N., Dinh, D. H., Olivero, W. C., Gujrati, M., Foster, D. C., ... Rao, J. S. (2003). Physiological and chemical inducers of tissue factor pathway inhibitor-2 in human glioma cells. International journal of oncology, 22(6), 1277-1283.

Physiological and chemical inducers of tissue factor pathway inhibitor-2 in human glioma cells. / Konduri, Santhi D.; Yanamandra, Niranjan; Dinh, Dzung H.; Olivero, William C.; Gujrati, Meena; Foster, Donald C.; Kisiel, Walter; Rao, Jasti S.

In: International journal of oncology, Vol. 22, No. 6, 01.01.2003, p. 1277-1283.

Research output: Contribution to journalArticle

Konduri, SD, Yanamandra, N, Dinh, DH, Olivero, WC, Gujrati, M, Foster, DC, Kisiel, W & Rao, JS 2003, 'Physiological and chemical inducers of tissue factor pathway inhibitor-2 in human glioma cells.', International journal of oncology, vol. 22, no. 6, pp. 1277-1283.
Konduri SD, Yanamandra N, Dinh DH, Olivero WC, Gujrati M, Foster DC et al. Physiological and chemical inducers of tissue factor pathway inhibitor-2 in human glioma cells. International journal of oncology. 2003 Jan 1;22(6):1277-1283.
Konduri, Santhi D. ; Yanamandra, Niranjan ; Dinh, Dzung H. ; Olivero, William C. ; Gujrati, Meena ; Foster, Donald C. ; Kisiel, Walter ; Rao, Jasti S. / Physiological and chemical inducers of tissue factor pathway inhibitor-2 in human glioma cells. In: International journal of oncology. 2003 ; Vol. 22, No. 6. pp. 1277-1283.
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