TY - JOUR
T1 - Physico-chemical model for DNA alkaline elution
T2 - New experimental evidence and differential role of DNA length, chain flexibility and superpacking
AU - Nicolini, C.
AU - Belmont, A.
AU - Zietz, S.
AU - Maura, A.
AU - Ping, A.
AU - Robbiano, L.
AU - Brambilla, G.
N1 - Funding Information:
This work was supported by CNR grants 80.01485.96 (Progetto Finalizzato “Controllo della Crescita Neoplastica”) and 79.02360.65. Claudio Nicolini was supported by a Researcha nd Study Leave Award from Temple University and by the National ResearchC ouncil, Italy.
PY - 1983/1/21
Y1 - 1983/1/21
N2 - For a better understanding of data provided by DNA alkaline elution technique, a new analytical model has been developed which takes into consideration both the physicochemical properties of in situ DNA strand (length and flexibility/superpacking) and the geometric and hydrodynamic configuration of the elution apparatus (flow and filter conditions). Simulation by this model of experimental data previously obtained before and after carcinogens administration, has shown that for constant flow and filter conditions elution profiles are dependent, not only from DNA molecular weight, but also from a parameter critically related to modifications in chain flexibility/superpacking. This has been confirmed by several independent observations, including the time-dependent changes in non-denaturing lysing solution monitored by hydroxylapatite and alkaline elution techniques.
AB - For a better understanding of data provided by DNA alkaline elution technique, a new analytical model has been developed which takes into consideration both the physicochemical properties of in situ DNA strand (length and flexibility/superpacking) and the geometric and hydrodynamic configuration of the elution apparatus (flow and filter conditions). Simulation by this model of experimental data previously obtained before and after carcinogens administration, has shown that for constant flow and filter conditions elution profiles are dependent, not only from DNA molecular weight, but also from a parameter critically related to modifications in chain flexibility/superpacking. This has been confirmed by several independent observations, including the time-dependent changes in non-denaturing lysing solution monitored by hydroxylapatite and alkaline elution techniques.
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U2 - 10.1016/0022-5193(83)90357-0
DO - 10.1016/0022-5193(83)90357-0
M3 - Article
C2 - 6865448
AN - SCOPUS:0020693284
SN - 0022-5193
VL - 100
SP - 341
EP - 357
JO - Journal of Theoretical Biology
JF - Journal of Theoretical Biology
IS - 2
ER -