Phylogenomic analysis of the α proteasome gene family from early-diverging eukaryotes

Juan L. Bouzatrid, Leslie K. McNeilrid, Hugh M Robertson, Leellen F. Solter, Julie E. Nixon, Jonathan Edward Beever, H Rex Gaskins, Gary J Olsen, Shankar Subramaniam, Mitchell L. Sogin, Harris A. Lewinid

Research output: Contribution to journalArticle

Abstract

We employed a phylogenomic approach to study the evolution of α subunits of the proteasome gene family from early diverging eukaryotes. BLAST similarity searches of the Giardia lamblia genome identified all seven α proteasome genes characteristic of eukaryotes from the crown group. In addition, a PCR strategy for the amplification of multiple α subunit sequences generated single α proteasome products for representatives of the Kinetoplastida (Leishmania major), the Parabasalia (Trichomonas vaginalis), and the Microsporidia (Vairi-morpha sp., Nosema sp., Endoreticulata sp., and Spraguea lophii). The kinetoplastid Trypanosoma cruzi and the eukaryote crown group Acanthamoeba castellanii yielded two distinct α proteasome genes each. The presence of seven distinct α proteasome genes in G. lamblia, one of the earliest-diverging eukaryotes, indicates that the α proteasome gene family evolved rapidly from a minimum of one gene in Archaea to seven or more in Eukarya. Results from the phylogenomic analysis are consistent with the idea that the Diplomonida (as represented by G. lamblia), the Kinetoplastida, the Parabasalia, and the Microsporidia diverged after the duplication events that originated the α proteasome gene family. A model for the early origin and evolution of the proteasome gene family is presented.

Original languageEnglish (US)
Pages (from-to)532-543
Number of pages12
JournalJournal of Molecular Evolution
Volume51
Issue number6
StatePublished - 2000

Fingerprint

proteasome endopeptidase complex
eukaryote
Proteasome Endopeptidase Complex
Eukaryota
eukaryotic cells
Genes
gene
Giardia lamblia
Parabasalidea
Kinetoplastida
genes
Microsporidia
Crowns
Nosema
Acanthamoeba castellanii
Trichomonas vaginalis
Leishmania major
analysis
family
Trypanosoma cruzi

Keywords

  • Duplications
  • Gene families
  • Orthologues
  • Paraiogues
  • Phylogenomics
  • Proteasome

ASJC Scopus subject areas

  • Genetics
  • Biochemistry
  • Biochemistry, Genetics and Molecular Biology(all)
  • Genetics(clinical)
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Agricultural and Biological Sciences(all)
  • Agricultural and Biological Sciences (miscellaneous)

Cite this

Bouzatrid, J. L., McNeilrid, L. K., Robertson, H. M., Solter, L. F., Nixon, J. E., Beever, J. E., ... Lewinid, H. A. (2000). Phylogenomic analysis of the α proteasome gene family from early-diverging eukaryotes. Journal of Molecular Evolution, 51(6), 532-543.

Phylogenomic analysis of the α proteasome gene family from early-diverging eukaryotes. / Bouzatrid, Juan L.; McNeilrid, Leslie K.; Robertson, Hugh M; Solter, Leellen F.; Nixon, Julie E.; Beever, Jonathan Edward; Gaskins, H Rex; Olsen, Gary J; Subramaniam, Shankar; Sogin, Mitchell L.; Lewinid, Harris A.

In: Journal of Molecular Evolution, Vol. 51, No. 6, 2000, p. 532-543.

Research output: Contribution to journalArticle

Bouzatrid, JL, McNeilrid, LK, Robertson, HM, Solter, LF, Nixon, JE, Beever, JE, Gaskins, HR, Olsen, GJ, Subramaniam, S, Sogin, ML & Lewinid, HA 2000, 'Phylogenomic analysis of the α proteasome gene family from early-diverging eukaryotes', Journal of Molecular Evolution, vol. 51, no. 6, pp. 532-543.
Bouzatrid JL, McNeilrid LK, Robertson HM, Solter LF, Nixon JE, Beever JE et al. Phylogenomic analysis of the α proteasome gene family from early-diverging eukaryotes. Journal of Molecular Evolution. 2000;51(6):532-543.
Bouzatrid, Juan L. ; McNeilrid, Leslie K. ; Robertson, Hugh M ; Solter, Leellen F. ; Nixon, Julie E. ; Beever, Jonathan Edward ; Gaskins, H Rex ; Olsen, Gary J ; Subramaniam, Shankar ; Sogin, Mitchell L. ; Lewinid, Harris A. / Phylogenomic analysis of the α proteasome gene family from early-diverging eukaryotes. In: Journal of Molecular Evolution. 2000 ; Vol. 51, No. 6. pp. 532-543.
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