Phylogenetic Origin of the Chloroplast

Research output: Contribution to journalArticle

Abstract

SYNOPSIS. The 16S ribosomal RNA of the chloroplast of Euglena gracilis strain Z has been characterized in terms of its 2‐dimensional electrophoretic “fingerprint” (T1 ribonuclease). Over 100 spots were resolved on the “fingerprint” and each spot was characterized as to which RNA oligonucleotide fragment(s) it contained. When compared to similar analyses of prokaryotic 16S rRNAs and eukaryotic cytoplasmic 18S rRNAs, the chloroplast 16S rRNA was a typically prokaryotic RNA, but bore little if any relationship to eukaryotic 18S rRNAs. Therefore, the cistrons for chloroplast 16S rRNA are related to the equivalent prokaryotic cistrons, but, apparently, are not related to the equivalent eukaryotic cistrons. Among the organisms available for comparison, the Euglena chloroplast 16S rRNA appears most closely related to the 16S rRNA of the eukaryote, Porphyridium cruentum (a red alga), and at least distantly related to the 16S rRNAs of the blue‐green algae and perhaps also to the bacilli.

Original languageEnglish (US)
Pages (from-to)41-47
Number of pages7
JournalThe Journal of Protozoology
Volume23
Issue number1
DOIs
StatePublished - Feb 1976

Keywords

  • 16S ribosomal RNA
  • Euglena gracilis strain Z
  • chloroplast
  • electrophoretic “fingerprint”
  • eukaryote
  • phylogeny
  • prokaryote

ASJC Scopus subject areas

  • Parasitology

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