Phylogenetic evidence for a fusion of archaeal and bacterial SemiSWEETs to form eukaryotic SWEETs and identification of SWEET hexose transporters in the amphibian chytrid pathogen Batrachochytrium dendrobatidis

Yi Bing Hu, Davide Sosso, Xiao Qing Qu, Li Qing Chen, Lai Ma, Diane Chermak, De Chun Zhang, Wolf B. Frommer

Research output: Contribution to journalArticle

Abstract

SWEETs represent a new class of sugar transporters first described in plants, animals, and humans and later in prokaryotes. PlantSWEETs play key roles in phloemloading, seed filling, and nectar secretion,whereas the role of archaeal, bacterial, and animal transporters remains elusive. Structural analyses show that eukaryotic SWEETs are composed of 2 triple-helix bundles (THBs) fused via an inversion linker helix, whereas prokaryotic SemiSWEETs contain only a single THB and require homodimerization to form transport pores. This study indicates that SWEETs retained sugar transport activity in all kingdoms of life, and that SemiSWEETs are likely their ancestral units. Fusion of oligomeric subunits into single polypeptides during evolution of eukaryotes is commonly found for transporters. Phylogenetic analyses indicate that THBs of eukaryotic SWEETs may not have evolved by tandem duplication of an open reading frame, but rather originated by fusion between an archaeal and a bacterial SemiSWEET, which potentially explains the asymmetry of eukaryotic SWEETs. Moreover, despite the ancient ancestry, SWEETs had not been identified in fungi or oomycetes. Here, we report the identification of SWEETs in oomycetes as well as SWEETs and a potential SemiSWEET in primitive fungi. BdSWEET1 and BdSWEET2 from Batrachochytrium dendrobatidis, a nonhyphal zoosporic fungus that causes global decline in amphibians, showed glucose and fructose transport activities.

Original languageEnglish (US)
Pages (from-to)3644-3654
Number of pages11
JournalFASEB Journal
Volume30
Issue number10
DOIs
StatePublished - Oct 2016

Fingerprint

Chytridiomycota
Monosaccharide Transport Proteins
Amphibians
Pathogens
Fungi
Oomycetes
Fusion reactions
Sugars
Animals
Plant Nectar
Fructose
Eukaryota
Open Reading Frames
Seed
Seeds
Glucose
Peptides

Keywords

  • Gene Conversion
  • LECA
  • Oomycete
  • Sugar Transport
  • Transporter Evolution

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

Cite this

Phylogenetic evidence for a fusion of archaeal and bacterial SemiSWEETs to form eukaryotic SWEETs and identification of SWEET hexose transporters in the amphibian chytrid pathogen Batrachochytrium dendrobatidis. / Hu, Yi Bing; Sosso, Davide; Qu, Xiao Qing; Chen, Li Qing; Ma, Lai; Chermak, Diane; Zhang, De Chun; Frommer, Wolf B.

In: FASEB Journal, Vol. 30, No. 10, 10.2016, p. 3644-3654.

Research output: Contribution to journalArticle

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AU - Sosso, Davide

AU - Qu, Xiao Qing

AU - Chen, Li Qing

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AU - Chermak, Diane

AU - Zhang, De Chun

AU - Frommer, Wolf B.

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