Phylogenetic characterization of Encephalitozoon romaleae (Microsporidia) from a grasshopper host: Relationship to Encephalitozoon spp. infecting humans

Shajahan Johny, Troy M. Larson, Leellen F. Solter, Kevin A. Edwards, Douglas W. Whitman

Research output: Contribution to journalArticle

Abstract

Encephalitozoon species are the most common microsporidian pathogens of humans and domesticated animals. We recently discovered a new microsporidium, Encephalitozoon romaleae, infecting the eastern lubber grasshopper Romalea microptera. To understand its evolutionary relationships, we compared partial gene sequences of α- and β-tubulin and methionine aminopeptidase 2 enzyme from this and related species. We also analyzed the rRNA internal transcribed spacer. Based on tubulin and MetAP-2 gene phylogenetic analysis, E. romaleae clustered with the Encephalitzoon group with strong bootstrap support (>99%). Within the Encephalitozoon clade, E. romaleae clustered with Encephalitozoon hellem for both the β-tubulin and MetAP-2 phylogenies based on ML tree. The α-tubulin based ML tree, however, placed the new microsporidium closer to Encephalitozoon cuniculi. The rRNA internal transcribed spacer region of E. romaleae has 91% homology with E. hellem.

Original languageEnglish (US)
Pages (from-to)189-195
Number of pages7
JournalInfection, Genetics and Evolution
Volume9
Issue number2
DOIs
StatePublished - Mar 1 2009

Keywords

  • Encephalitozoon
  • Grasshopper
  • Methionine aminopeptidase 2
  • Microsporidia
  • Phylogeny
  • Romalea microptera
  • Tubulin gene
  • rRNA

ASJC Scopus subject areas

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics
  • Molecular Biology
  • Genetics
  • Microbiology (medical)
  • Infectious Diseases

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