Phylogenetic analysis of the pPT23A plasmid family of Pseudomonas syringae

Zhonghua Ma, James J. Smith, Youfu Zhao, Robert W. Jackson, Dawn L. Arnold, Jesús Murillo, George W. Sundin

Research output: Contribution to journalArticlepeer-review

Abstract

The pPT23A plasmid family of Pseudomonas syringae contains members that contribute to the ecological and pathogenic fitness of their P. syringae hosts. In an effort to understand the evolution of these plasmids and their hosts, we undertook a comparative analysis of the phylogeny of plasmid genes and that of conserved chromosomal genes from P. syringae. In total, comparative sequence and phylogenetic analyses were done utilizing 47 pPT23A family plasmids (PFPs) from 16 pathovars belonging to six genomospecies. Our results showed that the plasmid replication gene (repA), the only gene currently known to be distributed among all the PFPs, had a phylogeny that was distinct from that of the P. syringae hosts of these plasmids and from those of other individual genes on PFPs. The phylogenies of two housekeeping chromosomal genes, those for DNA gyrase B subunit (gyrB) and primary sigma factor (rpoD), however, were strongly associated with genomospecies of P. syringae. Based on the results from this study, we conclude that the pPT23A plasmid family represents a dynamic genome that is mobile among P. syringae pathovars.

Original languageEnglish (US)
Pages (from-to)1287-1295
Number of pages9
JournalApplied and environmental microbiology
Volume73
Issue number4
DOIs
StatePublished - Feb 2007
Externally publishedYes

ASJC Scopus subject areas

  • Biotechnology
  • Food Science
  • Ecology
  • Applied Microbiology and Biotechnology

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