TY - GEN
T1 - Photothermal bleaching in time-lapse photoacoustic microscopy
AU - Gao, Liang
AU - Wang, Lidai
AU - Li, Chiye
AU - Garcia-Uribe, Alejandro
AU - Wang, Lihong V.
PY - 2014/3/3
Y1 - 2014/3/3
N2 - We studied the phenomenon of photothermal bleaching - a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time-lapse imaging applications.
AB - We studied the phenomenon of photothermal bleaching - a gradual reduction of contrast agent particles during repeated scans in photoacoustic microscopy. The dependence of the photothermal bleaching rate on the excitation pulse energy was determined while the laser focal diameter was held constant. Our results showed that, the dependence of the photothermal bleaching rate on the excitation pulse energy differed before and after the absorbers were raised to their melting point by the deposited laser energy. Based on this finding, we suggested an optimal excitation pulse energy, which balances the photothermal bleaching rate and signal amplitude, for time-lapse imaging applications.
KW - Photoacoustic microscopy
KW - Photothermal bleaching
UR - http://www.scopus.com/inward/record.url?scp=84902097030&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84902097030&partnerID=8YFLogxK
U2 - 10.1117/12.2038564
DO - 10.1117/12.2038564
M3 - Conference contribution
AN - SCOPUS:84902097030
SN - 9780819498564
T3 - Progress in Biomedical Optics and Imaging - Proceedings of SPIE
BT - Photons Plus Ultrasound
PB - SPIE
T2 - Photons Plus Ultrasound: Imaging and Sensing 2014
Y2 - 2 February 2014 through 5 February 2014
ER -