Photothermal bleaching and recovery analysis in photoacoustic microscopy

Chiye Li, Chi Zhang, Liang Gao, Alejandro Garcia-Uribe, Lihong V. Wang

Research output: Chapter in Book/Report/Conference proceedingConference contribution


A novel method - photoacoustic recovery after photothermal bleaching (PRAP) - is proposed and implemented to study particle dynamics and medium properties at the micron scale via photoacoustic imaging. PRAP is an intuitive way to visualize as well as quantify dynamic processes in many kinds of media. We demonstrate PRAP first in a phantom study, and then in live cells. PRAP provides high signal-to-noise ratio imaging with minimal bleaching-induced artifacts during the recovery stage, ideal for monitoring the diffusive and kinetic phenomena inside a cell.

Original languageEnglish (US)
Title of host publicationPhotons Plus Ultrasound
Subtitle of host publicationImaging and Sensing 2014
ISBN (Print)9780819498564
StatePublished - 2014
EventPhotons Plus Ultrasound: Imaging and Sensing 2014 - San Francisco, CA, United States
Duration: Feb 2 2014Feb 5 2014

Publication series

NameProgress in Biomedical Optics and Imaging - Proceedings of SPIE
ISSN (Print)1605-7422


OtherPhotons Plus Ultrasound: Imaging and Sensing 2014
CountryUnited States
CitySan Francisco, CA


  • Diffusion
  • Kinetics
  • Nanoparticle
  • Photoacoustic microscopy
  • Photothermal bleaching

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Atomic and Molecular Physics, and Optics
  • Biomaterials
  • Radiology Nuclear Medicine and imaging

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