Interferometric scattering microscopy is a newly emerging alternative to fluorescence microscopy in biomedical research and diagnostic testing due to its ability to detect nano-objects such as individual proteins, extracellular vesicles, and virions individually through their intrinsic elastic light scattering. To improve the signal-to-noise ratio, we developed photonic resonator interferometric scattering microscopy (PRISM) in which a photonic crystal (PC) resonator is used as the sample substrate. The scattered light is amplified by the PC through resonant near-field enhancement, which then interferes with the <1% transmitted light to create intensity contrast. Importantly, the scattered photons assume the wavevectors defined by PC's photonic band structure, resulting in the ability to utilize a non-immersion objective without significant loss at illumination density as low as 25 W/cm2. We demonstrate virus and protein detection, including highly selective capture and counting of intact pseudotype SARS-CoV-2 from saliva with sensitivity equivalent to conventional nucleic acid tests. The results showcase the promise of nanophotonic surfaces in the development of resonance-enhanced interferometric microscopies, and as a single step, room temperature, and rapid viral detection technology.

Original languageEnglish (US)
Title of host publicationIntegrated Optics
Subtitle of host publicationDevices, Materials, and Technologies XXVI
EditorsSonia M. Garcia-Blanco, Pavel Cheben
ISBN (Electronic)9781510648791
StatePublished - 2022
EventIntegrated Optics: Devices, Materials, and Technologies XXVI 2022 - Virtual, Online
Duration: Feb 20 2022Feb 24 2022

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
ISSN (Print)0277-786X
ISSN (Electronic)1996-756X


ConferenceIntegrated Optics: Devices, Materials, and Technologies XXVI 2022
CityVirtual, Online


  • SARS-CoV-2
  • diagnostics
  • interferometric scattering
  • label-free
  • photonic crystal

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering


Dive into the research topics of 'Photonic resonator interferometric scattering microscopy'. Together they form a unique fingerprint.

Cite this