TY - GEN
T1 - Photonic crystals utilized for label-free and amplified fluorescence biodetection
AU - Cunningham, Brian T.
PY - 2008
Y1 - 2008
N2 - Photonic crystals are fabricated on plastic surfaces, producing narrow bandwidth resonances at any desired wavelength. While shifts in the resonant wavelength quantify the density of adsorbed biomaterial, the resonances also enhance the output of adsorbed fluorophores. The combined attributes of photonic crystals enable highly sensitive label-free detection and greatly amplified sensitivity of any fluorescence-based assay for applications in life science research, drug discovery, and environmental pathogen detection. Applications for label-free selective detection of viral pathogens with sensitivity of ∼30 focal forming units, and detection of spore pathogens with single-unit resolution are highlighted. Amplified fluorescence, meanwhile, enables gene expression detection of DNA at concentrations ∼2 orders of magnitude lower than detection on optically passive surfaces.
AB - Photonic crystals are fabricated on plastic surfaces, producing narrow bandwidth resonances at any desired wavelength. While shifts in the resonant wavelength quantify the density of adsorbed biomaterial, the resonances also enhance the output of adsorbed fluorophores. The combined attributes of photonic crystals enable highly sensitive label-free detection and greatly amplified sensitivity of any fluorescence-based assay for applications in life science research, drug discovery, and environmental pathogen detection. Applications for label-free selective detection of viral pathogens with sensitivity of ∼30 focal forming units, and detection of spore pathogens with single-unit resolution are highlighted. Amplified fluorescence, meanwhile, enables gene expression detection of DNA at concentrations ∼2 orders of magnitude lower than detection on optically passive surfaces.
KW - Fluorescence enhancement
KW - Label-free biosensors
KW - Optical biosensors
KW - Photonic crystal
UR - https://www.scopus.com/pages/publications/44349162211
UR - https://www.scopus.com/pages/publications/44349162211#tab=citedBy
U2 - 10.1117/12.773626
DO - 10.1117/12.773626
M3 - Conference contribution
AN - SCOPUS:44349162211
SN - 9780819471505
T3 - Proceedings of SPIE - The International Society for Optical Engineering
BT - Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II
T2 - Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II
Y2 - 18 March 2008 through 20 March 2008
ER -