Photonic crystals utilized for label-free and amplified fluorescence biodetection

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

Photonic crystals are fabricated on plastic surfaces, producing narrow bandwidth resonances at any desired wavelength. While shifts in the resonant wavelength quantify the density of adsorbed biomaterial, the resonances also enhance the output of adsorbed fluorophores. The combined attributes of photonic crystals enable highly sensitive label-free detection and greatly amplified sensitivity of any fluorescence-based assay for applications in life science research, drug discovery, and environmental pathogen detection. Applications for label-free selective detection of viral pathogens with sensitivity of ∼30 focal forming units, and detection of spore pathogens with single-unit resolution are highlighted. Amplified fluorescence, meanwhile, enables gene expression detection of DNA at concentrations ∼2 orders of magnitude lower than detection on optically passive surfaces.

Original languageEnglish (US)
Title of host publicationMicro (MEMS) and Nanotechnologies for Space, Defense, and Security II
DOIs
StatePublished - Jun 2 2008
EventMicro (MEMS) and Nanotechnologies for Space, Defense, and Security II - Orlando, FL, United States
Duration: Mar 18 2008Mar 20 2008

Publication series

NameProceedings of SPIE - The International Society for Optical Engineering
Volume6959
ISSN (Print)0277-786X

Other

OtherMicro (MEMS) and Nanotechnologies for Space, Defense, and Security II
CountryUnited States
CityOrlando, FL
Period3/18/083/20/08

Fingerprint

Pathogens
Photonic crystals
Labels
Fluorescence
photonics
fluorescence
pathogens
crystals
Wavelength
Fluorophores
Gene expression
Biomaterials
Assays
DNA
Plastics
Bandwidth
spores
life sciences
gene expression
sensitivity

Keywords

  • Fluorescence enhancement
  • Label-free biosensors
  • Optical biosensors
  • Photonic crystal

ASJC Scopus subject areas

  • Electrical and Electronic Engineering
  • Condensed Matter Physics

Cite this

Cunningham, B. T. (2008). Photonic crystals utilized for label-free and amplified fluorescence biodetection. In Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II [695910] (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6959). https://doi.org/10.1117/12.773626

Photonic crystals utilized for label-free and amplified fluorescence biodetection. / Cunningham, Brian T.

Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II. 2008. 695910 (Proceedings of SPIE - The International Society for Optical Engineering; Vol. 6959).

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Cunningham, BT 2008, Photonic crystals utilized for label-free and amplified fluorescence biodetection. in Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II., 695910, Proceedings of SPIE - The International Society for Optical Engineering, vol. 6959, Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II, Orlando, FL, United States, 3/18/08. https://doi.org/10.1117/12.773626
Cunningham BT. Photonic crystals utilized for label-free and amplified fluorescence biodetection. In Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II. 2008. 695910. (Proceedings of SPIE - The International Society for Optical Engineering). https://doi.org/10.1117/12.773626
Cunningham, Brian T. / Photonic crystals utilized for label-free and amplified fluorescence biodetection. Micro (MEMS) and Nanotechnologies for Space, Defense, and Security II. 2008. (Proceedings of SPIE - The International Society for Optical Engineering).
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