Photonic Crystal Enhanced Quantum Dot Biosensor for Cancer-associated miRNA Detection

Yanyu Xiong; Qinglan Huang; Taylor D. Canady; Priyash Barya; Shengyan Liu; Opeyemi H. Arogundade; Caitlin M. Race; Congnyu Che; Xiaojing Wang; Lifeng Zhou; Anh Igarashi; Xing Wang; Manish Kohli; Andrew M. Smith; Brian T. Cunningham

doi:10.1109/SENSORS52175.2022.9967032

Photonic Crystal Enhanced Quantum Dot Biosensor for Cancer-associated miRNA Detection

Yanyu Xiong, Qinglan Huang, Taylor D. Canady, Priyash Barya, Shengyan Liu, Opeyemi H. Arogundade, Caitlin M. Race, Congnyu Che, Xiaojing Wang, Lifeng Zhou, Anh Igarashi, Xing Wang, Manish Kohli, Andrew M. Smith, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Abstract

Nanoscale quantum emitters are useful tags for monitoring biomolecular interactions, but remain restricted by large numerical aperture objectives, blinking nature, and omnidirectional emission. Herein, using the multiplicative effects of amplified excitation, highly directed extraction, Purcell enhancement, and blinking suppression on a photonic crystal (PC) surface, we were able to boost the signal by over 3,000 times. Even with a low-NA lens (NA = 0.5, 50X) and an inexpensive optical setup, our platform provides single quantum dot (QD) sensitivity with a high signal-to-noise ratio (SNR = 59). By integrating the photonics enhancement with a newly designed bioassay, we achieved ultra sensitive detection with a detection limit up to 10aM and an over 9-log linear dose-response range using simple steps, room temperature workflow. In addition, we observed distinct surface motion trajectories of QDs when their surface attachment stringency is affected by a single base mutation in a cancer-specific miRNA sequence[1].

Original language	English (US)
Title of host publication	2022 IEEE Sensors, SENSORS 2022 - Conference Proceedings
Publisher	Institute of Electrical and Electronics Engineers Inc.
ISBN (Electronic)	9781665484640
DOIs	https://doi.org/10.1109/SENSORS52175.2022.9967032
State	Published - 2022
Event	2022 IEEE Sensors Conference, SENSORS 2022 - Dallas, United States Duration: Oct 30 2022 → Nov 2 2022

Publication series

Name	Proceedings of IEEE Sensors
Volume	2022-October
ISSN (Print)	1930-0395
ISSN (Electronic)	2168-9229

Conference

Conference	2022 IEEE Sensors Conference, SENSORS 2022
Country/Territory	United States
City	Dallas
Period	10/30/22 → 11/2/22

Keywords

Cancer Biomarker Detection
Photonic Crystal
Quantum Dot
miRNA

ASJC Scopus subject areas

Electrical and Electronic Engineering

Online availability

10.1109/SENSORS52175.2022.9967032

Library availability

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Cite this

Xiong, Y., Huang, Q., Canady, T. D., Barya, P., Liu, S., Arogundade, O. H., Race, C. M., Che, C., Wang, X., Zhou, L., Igarashi, A., Wang, X., Kohli, M., Smith, A. M., & Cunningham, B. T. (2022). Photonic Crystal Enhanced Quantum Dot Biosensor for Cancer-associated miRNA Detection. In 2022 IEEE Sensors, SENSORS 2022 - Conference Proceedings (Proceedings of IEEE Sensors; Vol. 2022-October). Institute of Electrical and Electronics Engineers Inc.. https://doi.org/10.1109/SENSORS52175.2022.9967032

Photonic Crystal Enhanced Quantum Dot Biosensor for Cancer-associated miRNA Detection. / Xiong, Yanyu; Huang, Qinglan; Canady, Taylor D. et al.
2022 IEEE Sensors, SENSORS 2022 - Conference Proceedings. Institute of Electrical and Electronics Engineers Inc., 2022. (Proceedings of IEEE Sensors; Vol. 2022-October).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution

Xiong, Y, Huang, Q, Canady, TD, Barya, P, Liu, S, Arogundade, OH, Race, CM, Che, C, Wang, X, Zhou, L, Igarashi, A, Wang, X, Kohli, M, Smith, AM & Cunningham, BT 2022, Photonic Crystal Enhanced Quantum Dot Biosensor for Cancer-associated miRNA Detection. in 2022 IEEE Sensors, SENSORS 2022 - Conference Proceedings. Proceedings of IEEE Sensors, vol. 2022-October, Institute of Electrical and Electronics Engineers Inc., 2022 IEEE Sensors Conference, SENSORS 2022, Dallas, United States, 10/30/22. https://doi.org/10.1109/SENSORS52175.2022.9967032

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title = "Photonic Crystal Enhanced Quantum Dot Biosensor for Cancer-associated miRNA Detection",

abstract = "Nanoscale quantum emitters are useful tags for monitoring biomolecular interactions, but remain restricted by large numerical aperture objectives, blinking nature, and omnidirectional emission. Herein, using the multiplicative effects of amplified excitation, highly directed extraction, Purcell enhancement, and blinking suppression on a photonic crystal (PC) surface, we were able to boost the signal by over 3,000 times. Even with a low-NA lens (NA = 0.5, 50X) and an inexpensive optical setup, our platform provides single quantum dot (QD) sensitivity with a high signal-to-noise ratio (SNR = 59). By integrating the photonics enhancement with a newly designed bioassay, we achieved ultra sensitive detection with a detection limit up to 10aM and an over 9-log linear dose-response range using simple steps, room temperature workflow. In addition, we observed distinct surface motion trajectories of QDs when their surface attachment stringency is affected by a single base mutation in a cancer-specific miRNA sequence[1].",

keywords = "Cancer Biomarker Detection, Photonic Crystal, Quantum Dot, miRNA",

author = "Yanyu Xiong and Qinglan Huang and Canady, {Taylor D.} and Priyash Barya and Shengyan Liu and Arogundade, {Opeyemi H.} and Race, {Caitlin M.} and Congnyu Che and Xiaojing Wang and Lifeng Zhou and Anh Igarashi and Xing Wang and Manish Kohli and Smith, {Andrew M.} and Cunningham, {Brian T.}",

note = "Publisher Copyright: {\textcopyright} 2022 IEEE.; 2022 IEEE Sensors Conference, SENSORS 2022 ; Conference date: 30-10-2022 Through 02-11-2022",

year = "2022",

doi = "10.1109/SENSORS52175.2022.9967032",

language = "English (US)",

series = "Proceedings of IEEE Sensors",

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AU - Xiong, Yanyu

AU - Huang, Qinglan

AU - Canady, Taylor D.

AU - Barya, Priyash

AU - Liu, Shengyan

AU - Arogundade, Opeyemi H.

AU - Race, Caitlin M.

AU - Che, Congnyu

AU - Wang, Xiaojing

AU - Zhou, Lifeng

AU - Igarashi, Anh

AU - Wang, Xing

AU - Kohli, Manish

AU - Smith, Andrew M.

AU - Cunningham, Brian T.

PY - 2022

Y1 - 2022

N2 - Nanoscale quantum emitters are useful tags for monitoring biomolecular interactions, but remain restricted by large numerical aperture objectives, blinking nature, and omnidirectional emission. Herein, using the multiplicative effects of amplified excitation, highly directed extraction, Purcell enhancement, and blinking suppression on a photonic crystal (PC) surface, we were able to boost the signal by over 3,000 times. Even with a low-NA lens (NA = 0.5, 50X) and an inexpensive optical setup, our platform provides single quantum dot (QD) sensitivity with a high signal-to-noise ratio (SNR = 59). By integrating the photonics enhancement with a newly designed bioassay, we achieved ultra sensitive detection with a detection limit up to 10aM and an over 9-log linear dose-response range using simple steps, room temperature workflow. In addition, we observed distinct surface motion trajectories of QDs when their surface attachment stringency is affected by a single base mutation in a cancer-specific miRNA sequence[1].

AB - Nanoscale quantum emitters are useful tags for monitoring biomolecular interactions, but remain restricted by large numerical aperture objectives, blinking nature, and omnidirectional emission. Herein, using the multiplicative effects of amplified excitation, highly directed extraction, Purcell enhancement, and blinking suppression on a photonic crystal (PC) surface, we were able to boost the signal by over 3,000 times. Even with a low-NA lens (NA = 0.5, 50X) and an inexpensive optical setup, our platform provides single quantum dot (QD) sensitivity with a high signal-to-noise ratio (SNR = 59). By integrating the photonics enhancement with a newly designed bioassay, we achieved ultra sensitive detection with a detection limit up to 10aM and an over 9-log linear dose-response range using simple steps, room temperature workflow. In addition, we observed distinct surface motion trajectories of QDs when their surface attachment stringency is affected by a single base mutation in a cancer-specific miRNA sequence[1].

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KW - Photonic Crystal

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KW - miRNA

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Photonic Crystal Enhanced Quantum Dot Biosensor for Cancer-associated miRNA Detection

Abstract

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Conference

Keywords

ASJC Scopus subject areas

Online availability

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