Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection

Anusha Pokhriyal, Meng Lu, Vikram Chaudhery, Cheng Sheng Huang, Stephen Schulz, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500× for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330× SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140× lower limit of detection compared to a conventional glass substrate.

Original languageEnglish (US)
Title of host publicationCLEO
Subtitle of host publicationScience and Innovations, CLEO_SI 2011
StatePublished - 2011
EventCLEO: Science and Innovations, CLEO_SI 2011 - Baltimore, MD, United States
Duration: May 1 2011May 6 2011

Other

OtherCLEO: Science and Innovations, CLEO_SI 2011
CountryUnited States
CityBaltimore, MD
Period5/1/115/6/11

Fingerprint

Photonic crystals
Quartz
quartz
Fluorescence
photonics
Nanoimprint lithography
fluorescence
Fluorophores
Polypeptides
Substrates
crystals
Dyes
polypeptides
Proteins
crystal surfaces
Glass
lithography
dyes
proteins
dosage

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

Cite this

Pokhriyal, A., Lu, M., Chaudhery, V., Huang, C. S., Schulz, S., & Cunningham, B. T. (2011). Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection. In CLEO: Science and Innovations, CLEO_SI 2011

Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection. / Pokhriyal, Anusha; Lu, Meng; Chaudhery, Vikram; Huang, Cheng Sheng; Schulz, Stephen; Cunningham, Brian T.

CLEO: Science and Innovations, CLEO_SI 2011. 2011.

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Pokhriyal, A, Lu, M, Chaudhery, V, Huang, CS, Schulz, S & Cunningham, BT 2011, Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection. in CLEO: Science and Innovations, CLEO_SI 2011. CLEO: Science and Innovations, CLEO_SI 2011, Baltimore, MD, United States, 5/1/11.
Pokhriyal A, Lu M, Chaudhery V, Huang CS, Schulz S, Cunningham BT. Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection. In CLEO: Science and Innovations, CLEO_SI 2011. 2011
Pokhriyal, Anusha ; Lu, Meng ; Chaudhery, Vikram ; Huang, Cheng Sheng ; Schulz, Stephen ; Cunningham, Brian T. / Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection. CLEO: Science and Innovations, CLEO_SI 2011. 2011.
@inproceedings{5654a1858e4f4b879133edd8c7578653,
title = "Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection",
abstract = "A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500× for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330× SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140× lower limit of detection compared to a conventional glass substrate.",
author = "Anusha Pokhriyal and Meng Lu and Vikram Chaudhery and Huang, {Cheng Sheng} and Stephen Schulz and Cunningham, {Brian T.}",
year = "2011",
language = "English (US)",
isbn = "9781557529107",
booktitle = "CLEO",

}

TY - GEN

T1 - Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection

AU - Pokhriyal, Anusha

AU - Lu, Meng

AU - Chaudhery, Vikram

AU - Huang, Cheng Sheng

AU - Schulz, Stephen

AU - Cunningham, Brian T.

PY - 2011

Y1 - 2011

N2 - A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500× for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330× SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140× lower limit of detection compared to a conventional glass substrate.

AB - A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500× for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330× SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140× lower limit of detection compared to a conventional glass substrate.

UR - http://www.scopus.com/inward/record.url?scp=84893614230&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=84893614230&partnerID=8YFLogxK

M3 - Conference contribution

AN - SCOPUS:84893614230

SN - 9781557529107

BT - CLEO

ER -