Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection

Anusha Pokhriyal, Meng Lu, Vikram Chaudhery, Cheng Sheng Huang, Stephen Schulz, Brian T. Cunningham

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

A Photonic Crystal surface fabricated upon a quartz substrate using nanoimprint lithography has been demonstrated with a signal enhancement factor as high as 7500× for the detection of LD-700 dye spin-coated upon the PC. The detection of spotted Alexa-647 labeled polypeptide on the PC exhibits a 330× SNR improvement. Using dose-response characterization of deposited fluorophore-tagged protein spots, the PCEF surface demonstrated a 140× lower limit of detection compared to a conventional glass substrate.

Original languageEnglish (US)
Title of host publicationCLEO
Subtitle of host publicationScience and Innovations, CLEO_SI 2011
StatePublished - 2011
EventCLEO: Science and Innovations, CLEO_SI 2011 - Baltimore, MD, United States
Duration: May 1 2011May 6 2011

Other

OtherCLEO: Science and Innovations, CLEO_SI 2011
CountryUnited States
CityBaltimore, MD
Period5/1/115/6/11

ASJC Scopus subject areas

  • Instrumentation
  • Atomic and Molecular Physics, and Optics

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  • Cite this

    Pokhriyal, A., Lu, M., Chaudhery, V., Huang, C. S., Schulz, S., & Cunningham, B. T. (2011). Photonic crystal enhanced fluorescence using a quartz substrate to reduce limits of detection. In CLEO: Science and Innovations, CLEO_SI 2011