TY - JOUR
T1 - Photonic crystal enhanced fluorescence emission and blinking suppression for single quantum dot digital resolution biosensing
AU - Wang, Xing
N1 - This work is supported by the National Institutes of Health (NIH) R01-GM108584 (B.T.C.), R01-CA227699 (A.M.S., B.T.C., and M.K.), R01-CA212097 (M.K.), National Science Foundation (NSF) CBET-1900277 (B.T.C.), the Carl Woese Institute for Genomic Biology postdoctoral fellowship (T.C. and L.Z.), and the Cancer Center at Illinois for the C*STAR research fellowship (Y.X.) and TiME fellowship (O.H.A.). The authors gratefully acknowledge G.A. Fried, G. Popescu, J.A.N.T. Soares, P.R. Selvin, S. Sarkar, O.R. Teniola, D.E. Vaz, L.E. Kwon, J. Tibbs, S. Shepherd, B.K. Maity, A.J. Cyphersmith, E. Araud, C.-W. Kuo, Y. Zhuo, P. Le, F.-C. Hsiao, L.D. Akin, S. Nalla, N. Chauhan, A. Igarashi, and the rest of Nanosensor group for valuable discussions. The authors thank H. Zhou, J.C. Spear, K.A. Walsh, W. Swiech, and K.M. Flatt from Materials Research Lab at the University of Illinois at Urbana-Champaign for their training and support, and W. Wang for the assistance in coverslip cleaning. The authors also acknowledge MOXTEK, Inc. and Y. Yan for providing the SEM images of the grating structure.
This work is supported by the National Institutes of Health (NIH) R01-GM108584 (B.T.C.), R01-CA227699 (A.M.S., B.T.C., and M.K.), R01-CA212097 (M.K.), National Science Foundation (NSF) CBET-1900277 (B.T.C.), the Carl Woese Institute for Genomic Biology postdoctoral fellowship (T.C. and L.Z.), and the Cancer Center at Illinois for the C*STAR research fellowship (Y.X.) and TiME fellowship (O.H.A.). The authors gratefully acknowledge G.A. Fried, G. Popescu, J.A.N.T. Soares, P.R. Selvin, S. Sarkar, O.R. Teniola, D.E. Vaz, L.E. Kwon, J. Tibbs, S. Shepherd, B.K. Maity, A.J. Cyphersmith, E. Araud, C.-W. Kuo, Y. Zhuo, P. Le, F.-C. Hsiao, L.D. Akin, S. Nalla, N. Chauhan, A. Igarashi, and the rest of Nanosensor group for valuable discussions. The authors thank H. Zhou, J.C. Spear, K.A. Walsh, W. Swiech, and K.M. Flatt from Materials Research Lab at the University of Illinois at Urbana-Champaign for their training and support, and W. Wang for the assistance in coverslip cleaning. The authors also acknowledge MOXTEK, Inc. and Y. Yan for providing the SEM images of the grating structure.
PY - 2022/8/8
Y1 - 2022/8/8
N2 - While nanoscale quantum emitters are effective tags for measuring biomolecular interactions, their utilities for applications that demand single-unit observations are limited by the requirements for large numerical aperture (NA) objectives, fluorescence intermittency, and poor photon collection efficiency resulted from omnidirectional emission. Here, we report a nearly 3000-fold signal enhancement achieved through multiplicative effects of enhanced excitation, highly directional extraction, quantum efficiency improvement, and blinking suppression through a photonic crystal (PC) surface. The approach achieves single quantum dot (QD) sensitivity with high signal-to-noise ratio, even when using a low-NA lens and an inexpensive optical setup. The blinking suppression capability of the PC improves the QDs on-time from 15% to 85% ameliorating signal intermittency. We developed an assay for cancer-associated miRNA biomarkers with single-molecule resolution, single-base mutation selectivity, and 10-attomolar detection limit. Additionally, we observed differential surface motion trajectories of QDs when their surface attachment stringency is altered by changing a single base in a cancer-specific miRNA sequence.
AB - While nanoscale quantum emitters are effective tags for measuring biomolecular interactions, their utilities for applications that demand single-unit observations are limited by the requirements for large numerical aperture (NA) objectives, fluorescence intermittency, and poor photon collection efficiency resulted from omnidirectional emission. Here, we report a nearly 3000-fold signal enhancement achieved through multiplicative effects of enhanced excitation, highly directional extraction, quantum efficiency improvement, and blinking suppression through a photonic crystal (PC) surface. The approach achieves single quantum dot (QD) sensitivity with high signal-to-noise ratio, even when using a low-NA lens and an inexpensive optical setup. The blinking suppression capability of the PC improves the QDs on-time from 15% to 85% ameliorating signal intermittency. We developed an assay for cancer-associated miRNA biomarkers with single-molecule resolution, single-base mutation selectivity, and 10-attomolar detection limit. Additionally, we observed differential surface motion trajectories of QDs when their surface attachment stringency is altered by changing a single base in a cancer-specific miRNA sequence.
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UR - http://www.scopus.com/inward/citedby.url?scp=85135549914&partnerID=8YFLogxK
U2 - 10.1038/s41467-022-32387-w
DO - 10.1038/s41467-022-32387-w
M3 - Article
C2 - 35941132
SN - 2041-1723
VL - 13
JO - Nature communications
JF - Nature communications
IS - 1
M1 - 4647
ER -