Photon scanning tunneling microscopy of unstained mammalian cells and chromosomes

Fabrice Meriaudeau, L. Stubbs, Jim E. Parks, K. Bruce Jacobson, Jean Pierre Goudonnet, Trinidad L. Ferrell

Research output: Contribution to journalConference article

Abstract

The photon scanning tunneling microscope (PSTM) yields topographical images of samples which are thin or which are transparent at the wavelength used. A wide range of samples sizes may be imaged extending to well below the diffraction limit, but samples on the order of several to many micrometers in size can be analyzed if total internal reflection occur in the sample. We have used the PSTM to examine the topography of mouse and human cells and of mouse chromosomes that are unstained. Our objectives were to compare the images as an improved resolution alternative to phase contrast microscopy, and to provide a sample preparation methodology that will permit localized simultaneous fluorescence or absorption spectroscopy with the signal collected by the probe tip of the PSTM. Further, the PSTM's ability to produce topographical profiles was tested in order to establish the basis for future investigation of possible structural abnormalities in the chromosomes. That is, we had both physical and biological objectives to consider. To this end we utilized the 442 nm line of a HeCd laser, the resulting image quality being tested partly on order to ascertain the increase effects of Rayleigh scattering relative to results at longer wavelengths. It is shown that adequate resolution and signal-to-noise ration can be obtained with the shorter wavelength even in the presence of intensity fluctuations from the laser, thus showing that fluorescence and absorption can be expected to be practicable.

Original languageEnglish (US)
Pages (from-to)828-835
Number of pages8
JournalProceedings of SPIE - The International Society for Optical Engineering
Volume2782
DOIs
StatePublished - Dec 1 2004
EventOptical Inspection and Micromeasurements - Besancon, France
Duration: Jun 10 1996Jun 10 1996

Fingerprint

chromosomes
Scanning tunneling microscopy
Chromosomes
Microscopy
Chromosome
scanning tunneling microscopy
Scanning
Photon
Microscopes
Photons
Cells
Microscope
Wavelength
Cell
photons
microscopes
Mouse
Rayleigh scattering
Lasers
Fluorescence spectroscopy

Keywords

  • Biological samples
  • PSTM
  • Tunneling microscropy
  • chromosomes imaging
  • fluorescence
  • spectroscopy

ASJC Scopus subject areas

  • Electronic, Optical and Magnetic Materials
  • Condensed Matter Physics
  • Computer Science Applications
  • Applied Mathematics
  • Electrical and Electronic Engineering

Cite this

Photon scanning tunneling microscopy of unstained mammalian cells and chromosomes. / Meriaudeau, Fabrice; Stubbs, L.; Parks, Jim E.; Jacobson, K. Bruce; Goudonnet, Jean Pierre; Ferrell, Trinidad L.

In: Proceedings of SPIE - The International Society for Optical Engineering, Vol. 2782, 01.12.2004, p. 828-835.

Research output: Contribution to journalConference article

Meriaudeau, Fabrice ; Stubbs, L. ; Parks, Jim E. ; Jacobson, K. Bruce ; Goudonnet, Jean Pierre ; Ferrell, Trinidad L. / Photon scanning tunneling microscopy of unstained mammalian cells and chromosomes. In: Proceedings of SPIE - The International Society for Optical Engineering. 2004 ; Vol. 2782. pp. 828-835.
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