Pseudorabies virus was photoinactivated with a combination of methylene blue dye, light, and electricity. Viral suspensions were mixed with variable amounts of methylene blue dye and then were placed in a current source apparatus. Total inactivation of pseudorabies virus (1.7 x 106 50% tissue culture infective doses per ml) was achieved with constant mixing, a methylene blue dye concentration of 10-4 M, and an electrical current of 12 μA for 12 min.
ASJC Scopus subject areas
- Microbiology (medical)