Photodynamic inactivation of pseudorabies virus with methylene blue dye, light, and electricity

J. A. Badylak, G. Scherba, D. P. Gustafson

Research output: Contribution to journalArticlepeer-review

Abstract

Pseudorabies virus was photoinactivated with a combination of methylene blue dye, light, and electricity. Viral suspensions were mixed with variable amounts of methylene blue dye and then were placed in a current source apparatus. Total inactivation of pseudorabies virus (1.7 x 106 50% tissue culture infective doses per ml) was achieved with constant mixing, a methylene blue dye concentration of 10-4 M, and an electrical current of 12 μA for 12 min.

Original languageEnglish (US)
Pages (from-to)374-376
Number of pages3
JournalJournal of Clinical Microbiology
Volume17
Issue number2
DOIs
StatePublished - 1983

ASJC Scopus subject areas

  • Microbiology (medical)

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