PHOTOCLEAVAGE OF DNA: IRRADIATION OF QUINONE‐CONTAINING REAGENTS CONVERTS SUPERCOILED TO LINEAR DNA

Troels Koch, J. Dezz Ropp, Stephen G. Sligar, Gary B. Schuster

Research output: Contribution to journalArticle

Abstract

Irradiation (350 nm) of air‐saturated solutions of reagents containing an anthraquinone group linked to quaternary alkyl ammonium groups converts supercoiled DNA to circular and to linear DNA. Generation of linear DNA does not occur by accumulation of numerous single‐strand cuts but by coincident‐site double‐strand cleavage of DNA. Irradiation forms the triplet state of the anthraquinone, which reacts either by hydrogen atom abstraction from a sugar of DNA or by electron transfer from a base of the DNA. Subsequent reactions result in chain scission. The quinone is apparently reformed after this sequence and reirradiation leads to double‐strand cleavage.

Original languageEnglish (US)
Pages (from-to)554-558
Number of pages5
JournalPhotochemistry and Photobiology
Volume58
Issue number4
DOIs
StatePublished - Oct 1993

ASJC Scopus subject areas

  • Biochemistry
  • Physical and Theoretical Chemistry

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