PHOTOCLEAVAGE OF DNA: IRRADIATION OF QUINONE‐CONTAINING REAGENTS CONVERTS SUPERCOILED TO LINEAR DNA

Troels Koch; J. Dezz Ropp; Stephen G. Sligar; Gary B. Schuster

doi:10.1111/j.1751-1097.1993.tb04931.x

PHOTOCLEAVAGE OF DNA: IRRADIATION OF QUINONE‐CONTAINING REAGENTS CONVERTS SUPERCOILED TO LINEAR DNA

Troels Koch, J. Dezz Ropp, Stephen G. Sligar, Gary B. Schuster

Biochemistry

Research output: Contribution to journal › Article › peer-review

Abstract

Irradiation (350 nm) of air‐saturated solutions of reagents containing an anthraquinone group linked to quaternary alkyl ammonium groups converts supercoiled DNA to circular and to linear DNA. Generation of linear DNA does not occur by accumulation of numerous single‐strand cuts but by coincident‐site double‐strand cleavage of DNA. Irradiation forms the triplet state of the anthraquinone, which reacts either by hydrogen atom abstraction from a sugar of DNA or by electron transfer from a base of the DNA. Subsequent reactions result in chain scission. The quinone is apparently reformed after this sequence and reirradiation leads to double‐strand cleavage.

Original language	English (US)
Pages (from-to)	554-558
Number of pages	5
Journal	Photochemistry and Photobiology
Volume	58
Issue number	4
DOIs	https://doi.org/10.1111/j.1751-1097.1993.tb04931.x
State	Published - Oct 1993

ASJC Scopus subject areas

Biochemistry
Physical and Theoretical Chemistry

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10.1111/j.1751-1097.1993.tb04931.x

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abstract = "Irradiation (350 nm) of air‐saturated solutions of reagents containing an anthraquinone group linked to quaternary alkyl ammonium groups converts supercoiled DNA to circular and to linear DNA. Generation of linear DNA does not occur by accumulation of numerous single‐strand cuts but by coincident‐site double‐strand cleavage of DNA. Irradiation forms the triplet state of the anthraquinone, which reacts either by hydrogen atom abstraction from a sugar of DNA or by electron transfer from a base of the DNA. Subsequent reactions result in chain scission. The quinone is apparently reformed after this sequence and reirradiation leads to double‐strand cleavage.",

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T2 - IRRADIATION OF QUINONE‐CONTAINING REAGENTS CONVERTS SUPERCOILED TO LINEAR DNA

AU - Koch, Troels

AU - Ropp, J. Dezz

AU - Sligar, Stephen G.

AU - Schuster, Gary B.

PY - 1993/10

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AB - Irradiation (350 nm) of air‐saturated solutions of reagents containing an anthraquinone group linked to quaternary alkyl ammonium groups converts supercoiled DNA to circular and to linear DNA. Generation of linear DNA does not occur by accumulation of numerous single‐strand cuts but by coincident‐site double‐strand cleavage of DNA. Irradiation forms the triplet state of the anthraquinone, which reacts either by hydrogen atom abstraction from a sugar of DNA or by electron transfer from a base of the DNA. Subsequent reactions result in chain scission. The quinone is apparently reformed after this sequence and reirradiation leads to double‐strand cleavage.

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PHOTOCLEAVAGE OF DNA: IRRADIATION OF QUINONE‐CONTAINING REAGENTS CONVERTS SUPERCOILED TO LINEAR DNA

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