Phosphorylation of linker histone H1 regulates gene expression in vivo by mimicking H1 removal

Yali Dou, Craig A. Mizzen, Marc Abrams, C. David Allis, Martin A. Gorovsky

Research output: Contribution to journalArticlepeer-review

Abstract

Two Tetrahymena strains were created by gene replacement. One contained H1 with all phosphorylation sites mutated to alanine, preventing phosphorylation. The other had these sites changed to glutamic acid, mimicking the fully phosphorylated state. Global gene expression was not detectably changed in either strain. Instead, H1 phosphorylation activated or repressed specific genes in a manner that was remarkably similar to the effects of knocking out the gene encoding H1. These studies demonstrate a role for H1 phosphorylation in the regulation of transcription in vivo and suggest that it acts by mimicking the partial removal of H1.

Original languageEnglish (US)
Pages (from-to)641-647
Number of pages7
JournalMolecular cell
Volume4
Issue number4
DOIs
StatePublished - Oct 1999

ASJC Scopus subject areas

  • Molecular Biology
  • Cell Biology

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