Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena

Yi Wei, Craig A. Mizzen, Richard G. Cook, Martin A. Gorovsky, C. David Allis

Research output: Contribution to journalArticle

Abstract

H3 phosphorylation has been correlated with mitosis temporally in mammalian cells and spatially in ciliated protozoa. In logarithmically growing Tetrahymena thermophila cells, for example, H3 phosphorylation can be detected in germline micronuclei that divide mitotically but not in somatic macronuclei that divide amitotically. Here, we demonstrate that micronuclear H3 phosphorylation occurs at a single site (Ser-10) in the amino-terminal domain of histone H3, the same site phosphorylated during mitosis in mammalian cells. Using an antibody specific for Ser-10 phosphorylated H3, we show that, in Tetrahymena, this modification is correlated with mitotic and meiotic divisions of micronuclei in a fashion that closely coincides with chromosome condensation. Our data suggest that H3 phosphorylation at Ser-10 is a highly conserved event among eukaryotes and is likely involved in both mitotic and meiotic chromosome condensation.

Original languageEnglish (US)
Pages (from-to)7480-7484
Number of pages5
JournalProceedings of the National Academy of Sciences of the United States of America
Volume95
Issue number13
DOIs
StatePublished - Jun 23 1998

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Tetrahymena
Meiosis
Mitosis
Histones
Serine
Chromosomes
Phosphorylation
Germline Micronucleus
Macronucleus
Tetrahymena thermophila
Eukaryota
Antibodies

ASJC Scopus subject areas

  • General

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Phosphorylation of histone H3 at serine 10 is correlated with chromosome condensation during mitosis and meiosis in Tetrahymena. / Wei, Yi; Mizzen, Craig A.; Cook, Richard G.; Gorovsky, Martin A.; Allis, C. David.

In: Proceedings of the National Academy of Sciences of the United States of America, Vol. 95, No. 13, 23.06.1998, p. 7480-7484.

Research output: Contribution to journalArticle

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AU - Allis, C. David

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