Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi

María Laura Salto; Theresa Kuhlenschmidt; Mark Kuhlenschmidt; Rosa M. de Lederkremer; Roberto Docampo

doi:10.1016/j.molbiopara.2007.12.001

Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi

María Laura Salto, Theresa Kuhlenschmidt, Mark Kuhlenschmidt, Rosa M. de Lederkremer, Roberto Docampo

Pathobiology

Research output: Contribution to journal › Article › peer-review

Abstract

Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [³H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition.

Original language	English (US)
Pages (from-to)	120-130
Number of pages	11
Journal	Molecular and Biochemical Parasitology
Volume	158
Issue number	2
DOIs	https://doi.org/10.1016/j.molbiopara.2007.12.001
State	Published - Apr 2008

Keywords

Acidocalcisomes
Glycolipids
Phospholipids
Trypanosoma cruzi

ASJC Scopus subject areas

Parasitology
Molecular Biology

Online availability

10.1016/j.molbiopara.2007.12.001

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@article{88931b97fdcf4843b24021aac616e1d9,

title = "Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi",

abstract = "Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition.",

keywords = "Acidocalcisomes, Glycolipids, Phospholipids, Trypanosoma cruzi",

author = "Salto, {Mar{\'i}a Laura} and Theresa Kuhlenschmidt and Mark Kuhlenschmidt and {de Lederkremer}, {Rosa M.} and Roberto Docampo",

note = "This work was supported by a grant from the U.S. National Institutes of Health (AI68647) to R.D. This investigation was conducted in part in a facility constructed with support from Research Facility Improvement Grant Number C06 RR16515-01 from the National Center for Research Resources, National Institutes of Health.",

year = "2008",

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doi = "10.1016/j.molbiopara.2007.12.001",

language = "English (US)",

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AU - Salto, María Laura

AU - Kuhlenschmidt, Theresa

AU - Kuhlenschmidt, Mark

AU - de Lederkremer, Rosa M.

AU - Docampo, Roberto

N1 - This work was supported by a grant from the U.S. National Institutes of Health (AI68647) to R.D. This investigation was conducted in part in a facility constructed with support from Research Facility Improvement Grant Number C06 RR16515-01 from the National Center for Research Resources, National Institutes of Health.

PY - 2008/4

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N2 - Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition.

AB - Highly purified acidocalcisomes from Trypanosoma cruzi epimastigotes were obtained by differential centrifugation and iodixanol gradient ultracentrifugation. Lipid analysis of acidocalcisomes revealed the presence of low amounts of 3β-hydroxysterols and predominance of phospholipids. Alkylacyl phosphatidylinositol (16:0/18:2), diacyl phosphatidylinositol (18:0/18:2), diacyl phosphatidylcholine (16:0/18:2; 16:1/18:2; 16:2/18:2; 18:1/18:2 and 18:2/18:2), and diacyl phosphatidylethanolamine (16:0/18:2 and 16:1/18:2) were the only phospholipids characterized by electrospray ionization-mass spectrometry (ESI-MS). Incubation of epimastigotes with [3H]-mannose and isolation of acidocalcisomes allowed the detection of a glycoinositolphospholipid (GIPL) in these organelles. The sugar content of the acidocalcisomal GIPL was similar to that of the GIPL present in a microsomal fraction but the amount of galactofuranose and inositol with respect to the other monosaccharides was lower, suggesting a different chemical structure. Taken together, these results indicate that acidocalcisomes of T. cruzi have a distinct lipid and carbohydrate composition.

KW - Acidocalcisomes

KW - Glycolipids

KW - Phospholipids

KW - Trypanosoma cruzi

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VL - 158

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EP - 130

JO - Molecular and Biochemical Parasitology

JF - Molecular and Biochemical Parasitology

IS - 2

ER -

Phospholipid and glycolipid composition of acidocalcisomes of Trypanosoma cruzi

Abstract

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