Phenotypic and genotypic characterization of antibiotic-resistant soil and manure bacteria adjacent to swine production facilities

Melike Ekizoglu, Satoshi Koike, Ivan Krapac, Mahmut Nedim Sultan, Roderick Mackie

Research output: Contribution to journalArticlepeer-review

Abstract

This study aimed to identify phenotypic and genotypic resistance profiles of bacteria isolated from soil and manure samples obtained from a farm where tetracycline and tylosin were used extensively. Samples were collected from the manure and soil, before and after manure application. All of the 151 bacteria were identified based on BLAST results. Minimum inhibitory concentrations were determined by agar dilution and E-test methods. Class-specific primers were used to amplify 6 erythromycin and 7 tetracycline resistance (Tcr) genes for 46 tetracycline- and 18 erythromycin-resistant isolates. Tcr gene sequence identity between different species of isolates was identified to investigate the dissemination of resistance genes. All of the 151 bacteria belonged to 4 different phyla: Proteobacteria, Bacteroidetes, Actinobacteria, and Firmicutes. The majority of the tetracycline-resistant isolates were present in Pseudomonadaceae. Twenty of the isolates, representing 14 different genera, were positive for Tcr genes. Only 2 isolates possessed the erm(Q) gene. This study probably is the first to describe the presence of tetracycline genes in members of the genera Simplicispira and Agrococcus. Results of the study suggest that the diversity of Tcr gene-carrying bacteria are increasing. Furthermore, tetracycline-resistant bacteria could be detected 3 months after the manure application

Original languageEnglish (US)
Pages (from-to)504-511
Number of pages8
JournalTurkish Journal of Veterinary and Animal Sciences
Volume37
Issue number5
DOIs
StatePublished - 2013

Keywords

  • Antibiotic resistance
  • Erythromycin
  • Soil
  • Susceptibility
  • Swine manure
  • Tetracycline

ASJC Scopus subject areas

  • veterinary(all)

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