pH sensitivity of calmodulin distribution in nervous tissue fractions

Marlene A. Wilson, Rhanor Gillette

Research output: Contribution to journalArticle

Abstract

Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca2+ concentration and is pronounced at micromolar (near intracellular) levels of Ca2+. These data may relate to recent evidence that Ca2+/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.

Original languageEnglish (US)
Pages (from-to)190-193
Number of pages4
JournalBrain Research
Volume331
Issue number1
DOIs
StatePublished - Apr 1 1985

Fingerprint

Nerve Tissue
Calmodulin
Phosphoric Diester Hydrolases
Pleurobranchaea
Mollusca
Cyclic Nucleotides
Nervous System
Neurons

Keywords

  • Pleurobranchaea
  • calmodulin
  • pH
  • phosphodiesterase

ASJC Scopus subject areas

  • Developmental Biology
  • Molecular Biology
  • Clinical Neurology
  • Neuroscience(all)

Cite this

pH sensitivity of calmodulin distribution in nervous tissue fractions. / Wilson, Marlene A.; Gillette, Rhanor.

In: Brain Research, Vol. 331, No. 1, 01.04.1985, p. 190-193.

Research output: Contribution to journalArticle

@article{10e13d805c3142c7805f37ae49eb6d80,
title = "pH sensitivity of calmodulin distribution in nervous tissue fractions",
abstract = "Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca2+ concentration and is pronounced at micromolar (near intracellular) levels of Ca2+. These data may relate to recent evidence that Ca2+/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.",
keywords = "Pleurobranchaea, calmodulin, pH, phosphodiesterase",
author = "Wilson, {Marlene A.} and Rhanor Gillette",
year = "1985",
month = "4",
day = "1",
doi = "10.1016/0006-8993(85)90734-6",
language = "English (US)",
volume = "331",
pages = "190--193",
journal = "Brain Research",
issn = "0006-8993",
publisher = "Elsevier",
number = "1",

}

TY - JOUR

T1 - pH sensitivity of calmodulin distribution in nervous tissue fractions

AU - Wilson, Marlene A.

AU - Gillette, Rhanor

PY - 1985/4/1

Y1 - 1985/4/1

N2 - Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca2+ concentration and is pronounced at micromolar (near intracellular) levels of Ca2+. These data may relate to recent evidence that Ca2+/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.

AB - Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca2+ concentration and is pronounced at micromolar (near intracellular) levels of Ca2+. These data may relate to recent evidence that Ca2+/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.

KW - Pleurobranchaea

KW - calmodulin

KW - pH

KW - phosphodiesterase

UR - http://www.scopus.com/inward/record.url?scp=0022003764&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0022003764&partnerID=8YFLogxK

U2 - 10.1016/0006-8993(85)90734-6

DO - 10.1016/0006-8993(85)90734-6

M3 - Article

C2 - 3986563

AN - SCOPUS:0022003764

VL - 331

SP - 190

EP - 193

JO - Brain Research

JF - Brain Research

SN - 0006-8993

IS - 1

ER -