pH sensitivity of calmodulin distribution in nervous tissue fractions

Marlene A. Wilson; Rhanor Gillette

doi:10.1016/0006-8993(85)90734-6

pH sensitivity of calmodulin distribution in nervous tissue fractions

Research output: Contribution to journal › Article › peer-review

Abstract

Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca²⁺ concentration and is pronounced at micromolar (near intracellular) levels of Ca²⁺. These data may relate to recent evidence that Ca²⁺/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.

Original language	English (US)
Pages (from-to)	190-193
Number of pages	4
Journal	Brain Research
Volume	331
Issue number	1
DOIs	https://doi.org/10.1016/0006-8993(85)90734-6
State	Published - Apr 1 1985

Keywords

Pleurobranchaea
calmodulin
pH
phosphodiesterase

ASJC Scopus subject areas

General Neuroscience
Molecular Biology
Clinical Neurology
Developmental Biology

Online availability

10.1016/0006-8993(85)90734-6

Library availability

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title = "pH sensitivity of calmodulin distribution in nervous tissue fractions",

abstract = "Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca2+ concentration and is pronounced at micromolar (near intracellular) levels of Ca2+. These data may relate to recent evidence that Ca2+/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.",

keywords = "Pleurobranchaea, calmodulin, pH, phosphodiesterase",

author = "Wilson, {Marlene A.} and Rhanor Gillette",

note = "Funding Information: This research was supported by Grants NSF BNS 7918329 to R.G. and PHS-5-T32 GM07143 to M.A.W. Dr. K. Bonnet of the New York University School of Medicine contributed useful discussions.",

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AU - Gillette, Rhanor

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PY - 1985/4/1

Y1 - 1985/4/1

N2 - Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca2+ concentration and is pronounced at micromolar (near intracellular) levels of Ca2+. These data may relate to recent evidence that Ca2+/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.

AB - Alkalinization of nervous system extracts of the mollusk, Pleurobranchaea, from pH 7.0 to 8.0 markedly increases the ratio of soluble to total calmodulin. This effect is independent of pH effects on free Ca2+ concentration and is pronounced at micromolar (near intracellular) levels of Ca2+. These data may relate to recent evidence that Ca2+/calmodulin-activated cyclic nucleotide phosphodiesterase mediates the effects of small changes in intracellular pH (0.1-0.2 units) on the electrical activity of neurons. Calmodulin redistribution could reflect altered availability to stimulate phosphodiesterase activity and supports a a role for calmodulin in mediating effects of intracellular pH fluxes on cellular activity.

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pH sensitivity of calmodulin distribution in nervous tissue fractions

Abstract

Keywords

ASJC Scopus subject areas

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