Pervasive combinatorial modification of histone H3 in human cells

Benjamin A. Garcia, James J. Pesavento, Craig A. Mizzen, Neil L. Kelleher

Research output: Contribution to journalArticle

Abstract

We developed a platform using hydrophilic interaction chromatography and high-resolution tandem mass spectrometry (MS) for analyses of histone H3 that allows comprehensive characterization of 'histone codes' at the molecular level. We identified over 150 differentially modified forms of histone H3.2 in asynchronously grown and butyrate-treated HeLa cells, revealing pervasive combinatorial modification previously unaccounted for by other techniques and providing a clarified estimate of the molecular diversity of histone H3 in mammals.

Original languageEnglish (US)
Pages (from-to)487-489
Number of pages3
JournalNature Methods
Volume4
Issue number6
DOIs
StatePublished - Jun 1 2007

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Histones
Cells
Histone Code
Butyrates
Tandem Mass Spectrometry
Hydrophobic and Hydrophilic Interactions
HeLa Cells
Mammals
Chromatography
Mass spectrometry

ASJC Scopus subject areas

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Garcia, B. A., Pesavento, J. J., Mizzen, C. A., & Kelleher, N. L. (2007). Pervasive combinatorial modification of histone H3 in human cells. Nature Methods, 4(6), 487-489. https://doi.org/10.1038/nmeth1052

Pervasive combinatorial modification of histone H3 in human cells. / Garcia, Benjamin A.; Pesavento, James J.; Mizzen, Craig A.; Kelleher, Neil L.

In: Nature Methods, Vol. 4, No. 6, 01.06.2007, p. 487-489.

Research output: Contribution to journalArticle

Garcia, BA, Pesavento, JJ, Mizzen, CA & Kelleher, NL 2007, 'Pervasive combinatorial modification of histone H3 in human cells', Nature Methods, vol. 4, no. 6, pp. 487-489. https://doi.org/10.1038/nmeth1052
Garcia BA, Pesavento JJ, Mizzen CA, Kelleher NL. Pervasive combinatorial modification of histone H3 in human cells. Nature Methods. 2007 Jun 1;4(6):487-489. https://doi.org/10.1038/nmeth1052
Garcia, Benjamin A. ; Pesavento, James J. ; Mizzen, Craig A. ; Kelleher, Neil L. / Pervasive combinatorial modification of histone H3 in human cells. In: Nature Methods. 2007 ; Vol. 4, No. 6. pp. 487-489.
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