Peripheral non-opioid analgesic effects of kyotorphin in mice

Makoto Inoue, Hiroyuki Nakayamada, Shogo Tokuyama, Hiroshi Ueda

Research output: Contribution to journalArticlepeer-review

Abstract

Bradykinin (BK) given into the plantar (i.pl.) of the mouse hind-limb produced a flexor response. The flexor responses were dependent on BK doses (0.02-20 pmol, i.pl.), and were completely abolished by Hoe 140, a B2-type BK receptor antagonist. Kyotorphin, an analgesic neuropeptide which shows enkephalin release in brain slices, produced a dose-dependent reduction of the BK-induced nociceptive responses in ranges of 10 pmol to I nmol (i.pl.). Such analgesic effects of kyotorphin were reversed by leucine-arginine, a specific kyotorphin receptor antagonist, but not by naloxone. The kyotorphin- analgesia was also abolished by pertussis toxin (PTX) pretreatment. These results suggest that peripheral analgesic effects of kyotorphin are mediated through mechanisms of kyotorphin specific receptor and PTX-sensitive G(i)/G(o), and that the enkephalin release is not necessary for this analgesia.

Original languageEnglish (US)
Pages (from-to)60-62
Number of pages3
JournalNeuroscience Letters
Volume236
Issue number1
DOIs
StatePublished - Oct 24 1997
Externally publishedYes

Keywords

  • Bradykinin
  • G protein
  • Hoe 140
  • Kyotorphin
  • Leucine-arginine
  • Non-opioid
  • Peripheral analgesia
  • Pertussis toxin (PTX)

ASJC Scopus subject areas

  • Neuroscience(all)

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