Abstract
Objectives: Adequate choline supply during the perinatal period is critical for proper brain formation, when robust neurogenesis and neuronal maturation occur. Therefore, the objective of this study was to examine the impact of perinatal choline status on neurodevelopment. Methods: Sows were fed a choline-deficient (CD) or choline-sufficient (CS) diet during the last half of the gestational period. At 2 days of age, piglets from sows within each prenatal treatment group were further stratified into postnatal treatment groups and provided either a CD or CS milk replacer, resulting in four treatment groups. At 30 days of age, piglets underwent magnetic resonance imaging (MRI) procedures to analyze structural and metabolite differences. Results: Single-voxel spectroscopy (SVS) analysis revealed postnatally CS piglets had higher (P < 0.001) concentrations of glycerophosphocholine–phosphocholine than postnatally CD piglets. Volumetric analysis indicated smaller (P < 0.006) total brain volumes in prenatally CD piglets compared with prenatally CS piglets. Differences (P < 0.05) in the corpus callosum, pons, midbrain, thalamus, and right hippocampus, were observed as larger region-specific volumes proportional to total brain size in prenatally CD piglets compared with CS piglets. Diffusion tensor imaging (DTI) suggested interactions (P < 0.05) between prenatal and postnatal choline status in fractional anisotropy values of the thalamus and right hippocampus. Prenatally CS piglets had lower cerebellar radial diffusivity (P = 0.045) compared with prenatally CD piglets. Discussion: This study demonstrates that prenatal choline deficiency has profound effects by delaying neurodevelopment as evidenced by structural and metabolic MRI assessments.
| Original language | English (US) |
|---|---|
| Pages (from-to) | 425-433 |
| Number of pages | 9 |
| Journal | Nutritional Neuroscience |
| Volume | 19 |
| Issue number | 10 |
| DOIs | |
| State | Published - Dec 12 2016 |
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Keywords
- Choline
- Deficiency
- Diffusion tensor imaging
- Hippocampus
- Magnetic resonance imaging
- Perinatal
- Piglet
ASJC Scopus subject areas
- Neuroscience(all)
- Medicine (miscellaneous)
- Nutrition and Dietetics
Cite this
Perinatal choline deficiency delays brain development and alters metabolite concentrations in the young pig. / Mudd, Austin T.; Getty, Caitlyn M.; Sutton, Brad P.; Dilger, Ryan N.
In: Nutritional Neuroscience, Vol. 19, No. 10, 12.12.2016, p. 425-433.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Perinatal choline deficiency delays brain development and alters metabolite concentrations in the young pig
AU - Mudd, Austin T.
AU - Getty, Caitlyn M.
AU - Sutton, Brad P.
AU - Dilger, Ryan N.
PY - 2016/12/12
Y1 - 2016/12/12
N2 - Objectives: Adequate choline supply during the perinatal period is critical for proper brain formation, when robust neurogenesis and neuronal maturation occur. Therefore, the objective of this study was to examine the impact of perinatal choline status on neurodevelopment. Methods: Sows were fed a choline-deficient (CD) or choline-sufficient (CS) diet during the last half of the gestational period. At 2 days of age, piglets from sows within each prenatal treatment group were further stratified into postnatal treatment groups and provided either a CD or CS milk replacer, resulting in four treatment groups. At 30 days of age, piglets underwent magnetic resonance imaging (MRI) procedures to analyze structural and metabolite differences. Results: Single-voxel spectroscopy (SVS) analysis revealed postnatally CS piglets had higher (P < 0.001) concentrations of glycerophosphocholine–phosphocholine than postnatally CD piglets. Volumetric analysis indicated smaller (P < 0.006) total brain volumes in prenatally CD piglets compared with prenatally CS piglets. Differences (P < 0.05) in the corpus callosum, pons, midbrain, thalamus, and right hippocampus, were observed as larger region-specific volumes proportional to total brain size in prenatally CD piglets compared with CS piglets. Diffusion tensor imaging (DTI) suggested interactions (P < 0.05) between prenatal and postnatal choline status in fractional anisotropy values of the thalamus and right hippocampus. Prenatally CS piglets had lower cerebellar radial diffusivity (P = 0.045) compared with prenatally CD piglets. Discussion: This study demonstrates that prenatal choline deficiency has profound effects by delaying neurodevelopment as evidenced by structural and metabolic MRI assessments.
AB - Objectives: Adequate choline supply during the perinatal period is critical for proper brain formation, when robust neurogenesis and neuronal maturation occur. Therefore, the objective of this study was to examine the impact of perinatal choline status on neurodevelopment. Methods: Sows were fed a choline-deficient (CD) or choline-sufficient (CS) diet during the last half of the gestational period. At 2 days of age, piglets from sows within each prenatal treatment group were further stratified into postnatal treatment groups and provided either a CD or CS milk replacer, resulting in four treatment groups. At 30 days of age, piglets underwent magnetic resonance imaging (MRI) procedures to analyze structural and metabolite differences. Results: Single-voxel spectroscopy (SVS) analysis revealed postnatally CS piglets had higher (P < 0.001) concentrations of glycerophosphocholine–phosphocholine than postnatally CD piglets. Volumetric analysis indicated smaller (P < 0.006) total brain volumes in prenatally CD piglets compared with prenatally CS piglets. Differences (P < 0.05) in the corpus callosum, pons, midbrain, thalamus, and right hippocampus, were observed as larger region-specific volumes proportional to total brain size in prenatally CD piglets compared with CS piglets. Diffusion tensor imaging (DTI) suggested interactions (P < 0.05) between prenatal and postnatal choline status in fractional anisotropy values of the thalamus and right hippocampus. Prenatally CS piglets had lower cerebellar radial diffusivity (P = 0.045) compared with prenatally CD piglets. Discussion: This study demonstrates that prenatal choline deficiency has profound effects by delaying neurodevelopment as evidenced by structural and metabolic MRI assessments.
KW - Choline
KW - Deficiency
KW - Diffusion tensor imaging
KW - Hippocampus
KW - Magnetic resonance imaging
KW - Perinatal
KW - Piglet
UR - http://www.scopus.com/inward/record.url?scp=84978474495&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84978474495&partnerID=8YFLogxK
U2 - 10.1179/1476830515Y.0000000031
DO - 10.1179/1476830515Y.0000000031
M3 - Article
C2 - 26046479
AN - SCOPUS:84978474495
VL - 19
SP - 425
EP - 433
JO - Nutritional Neuroscience
JF - Nutritional Neuroscience
SN - 1028-415X
IS - 10
ER -