Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly

Hongdou Jia, Xiaobing Li, Guowen Liu, Juan J. Loor, Ryan Bucktrout, Xudong Sun, Guojin Li, Xin Shu, Jihong Dong, Yazhe Wang, Rankun Zuo, Zhe Wang, Xinwei Li

Research output: Contribution to journalArticle

Abstract

Fatty liver is a common metabolic disorder in dairy cows during the transition period. Perilipin 5 (PLIN5), a lipid droplet coat protein, plays important roles in the development of hepatic steatosis in mice and humans. Whether PLIN5 plays a role in the development of fatty liver in dairy cows is unknown. An in vivo study consisting of 10 healthy and 10 cows with fatty liver was performed to harvest liver tissue and blood samples. In addition, hepatocytes isolated from calves were infected with PLIN5 overexpression adenovirus for 48 h; treated with 0, 0.6, 1.2, or 2.4 mM nonesterified fatty acids (NEFA) for 24 h; or infected with PLIN5 silencing adenovirus for 48 h and then treated with 1.2 mM NEFA for 24 h. Serum concentrations of NEFA and β-hydroxybutyrate were greater in cows with fatty liver. Milk production and plasma glucose concentrations were lower in cows with fatty liver. The results revealed that PLIN5 is highly expressed in steatotic liver and localized to lipid droplets. The abundance of fatty acid and triacylglycerol (TAG) synthesis-related proteins including sterol regulatory element binding protein-1c, fatty acid synthase, acetyl-coA carboxylase 1, diacylglycerol acyltransferase 1, and diacylglycerol acyltransferase 2 was greater in the liver of cows with fatty liver. In contrast, the abundance of microsomal triglyceride transfer protein (MTP), apolipoprotein B100, and apolipoprotein E was lower in the liver of cows with fatty liver. Consequently, cows with fatty liver exhibited severe hepatic TAG accumulation and lower blood concentration of very low density lipoprotein apolipoprotein B (VLDL-ApoB). Overexpression of PLIN5 and exogenous NEFA in cultured hepatocytes increased the abundance of sterol regulatory element binding protein-1, fatty acid synthase, acetyl-coA carboxylase 1, diacylglycerol acyltransferase 1, and diacylglycerol acyltransferase 2 but decreased the abundance of microsomal triglyceride transfer protein, apolipoprotein B100, and apolipoprotein E, which promoted TAG synthesis and inhibited VLDL-ApoB assembly, inducing lipid accumulation. Importantly, knockdown of PLIN5 attenuated the upregulation of TAG synthesis and downregulation of VLDL-ApoB assembly induced by NEFA. Overall, these data suggest that NEFA activate PLIN5, leading to TAG accumulation and inhibition of VLDL assembly. As such, these mechanisms explain in part the development of hepatic steatosis in dairy cows.

Original languageEnglish (US)
Pages (from-to)833-845
Number of pages13
JournalJournal of Dairy Science
Volume102
Issue number1
DOIs
StatePublished - Jan 2019

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VLDL Lipoproteins
very low density lipoprotein
fatty liver
Fatty Liver
dairy cows
Diacylglycerol O-Acyltransferase
Nonesterified Fatty Acids
Lipids
synthesis
Liver
diacylglycerol acyltransferase
triacylglycerols
free fatty acids
lipids
Triglycerides
Apolipoproteins B
cows
apolipoprotein B
Sterol Regulatory Element Binding Protein 1
Fatty Acid Synthases

Keywords

  • dairy cow
  • fatty acid
  • fatty liver
  • perilipin 5

ASJC Scopus subject areas

  • Food Science
  • Animal Science and Zoology
  • Genetics

Cite this

Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. / Jia, Hongdou; Li, Xiaobing; Liu, Guowen; Loor, Juan J.; Bucktrout, Ryan; Sun, Xudong; Li, Guojin; Shu, Xin; Dong, Jihong; Wang, Yazhe; Zuo, Rankun; Wang, Zhe; Li, Xinwei.

In: Journal of Dairy Science, Vol. 102, No. 1, 01.2019, p. 833-845.

Research output: Contribution to journalArticle

Jia, H, Li, X, Liu, G, Loor, JJ, Bucktrout, R, Sun, X, Li, G, Shu, X, Dong, J, Wang, Y, Zuo, R, Wang, Z & Li, X 2019, 'Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly', Journal of Dairy Science, vol. 102, no. 1, pp. 833-845. https://doi.org/10.3168/jds.2018-15208
Jia, Hongdou ; Li, Xiaobing ; Liu, Guowen ; Loor, Juan J. ; Bucktrout, Ryan ; Sun, Xudong ; Li, Guojin ; Shu, Xin ; Dong, Jihong ; Wang, Yazhe ; Zuo, Rankun ; Wang, Zhe ; Li, Xinwei. / Perilipin 5 promotes hepatic steatosis in dairy cows through increasing lipid synthesis and decreasing very low density lipoprotein assembly. In: Journal of Dairy Science. 2019 ; Vol. 102, No. 1. pp. 833-845.
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AU - Bucktrout, Ryan

AU - Sun, Xudong

AU - Li, Guojin

AU - Shu, Xin

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AU - Li, Xinwei

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N2 - Fatty liver is a common metabolic disorder in dairy cows during the transition period. Perilipin 5 (PLIN5), a lipid droplet coat protein, plays important roles in the development of hepatic steatosis in mice and humans. Whether PLIN5 plays a role in the development of fatty liver in dairy cows is unknown. An in vivo study consisting of 10 healthy and 10 cows with fatty liver was performed to harvest liver tissue and blood samples. In addition, hepatocytes isolated from calves were infected with PLIN5 overexpression adenovirus for 48 h; treated with 0, 0.6, 1.2, or 2.4 mM nonesterified fatty acids (NEFA) for 24 h; or infected with PLIN5 silencing adenovirus for 48 h and then treated with 1.2 mM NEFA for 24 h. Serum concentrations of NEFA and β-hydroxybutyrate were greater in cows with fatty liver. Milk production and plasma glucose concentrations were lower in cows with fatty liver. The results revealed that PLIN5 is highly expressed in steatotic liver and localized to lipid droplets. The abundance of fatty acid and triacylglycerol (TAG) synthesis-related proteins including sterol regulatory element binding protein-1c, fatty acid synthase, acetyl-coA carboxylase 1, diacylglycerol acyltransferase 1, and diacylglycerol acyltransferase 2 was greater in the liver of cows with fatty liver. In contrast, the abundance of microsomal triglyceride transfer protein (MTP), apolipoprotein B100, and apolipoprotein E was lower in the liver of cows with fatty liver. Consequently, cows with fatty liver exhibited severe hepatic TAG accumulation and lower blood concentration of very low density lipoprotein apolipoprotein B (VLDL-ApoB). Overexpression of PLIN5 and exogenous NEFA in cultured hepatocytes increased the abundance of sterol regulatory element binding protein-1, fatty acid synthase, acetyl-coA carboxylase 1, diacylglycerol acyltransferase 1, and diacylglycerol acyltransferase 2 but decreased the abundance of microsomal triglyceride transfer protein, apolipoprotein B100, and apolipoprotein E, which promoted TAG synthesis and inhibited VLDL-ApoB assembly, inducing lipid accumulation. Importantly, knockdown of PLIN5 attenuated the upregulation of TAG synthesis and downregulation of VLDL-ApoB assembly induced by NEFA. Overall, these data suggest that NEFA activate PLIN5, leading to TAG accumulation and inhibition of VLDL assembly. As such, these mechanisms explain in part the development of hepatic steatosis in dairy cows.

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