Pericyte response to contraction mode-specific resistance exercise training in human skeletal muscle

Jean Farup, Michael De Lisio, Stine Klejs Rahbek, Jonas Bjerre, Mikkel Holm Vendelbo, Marni D. Boppart, Kristian Vissing

Research output: Contribution to journalArticlepeer-review

Abstract

Farup J, De Lisio M, Rahbek SK, Bjerre J, Vendelbo MH, Boppart MD, Vissing K. Pericyte response to contraction modespecific resistance exercise training in human skeletal muscle. J Appl Physiol 119: 1053-1063, 2015. First published September 24, 2015; doi:10.1152/japplphysiol.01108.2014.-Skeletal muscle satellite cells (SCs) are important for muscle repair and hypertrophy in response mechanical stimuli. Neuron-glial antigen 2-positive (NG2+) and alkaline phosphatase-positive (ALP+) pericytes may provide an alternative source of myogenic progenitors and/or secrete paracrine factors to induce Pax7+ SC proliferation and differentiation. The purpose of this study was to investigate NG2+ and ALP+ cell quantity, as well as SC content and activation, in human skeletal muscle following prolonged concentric (Conc) or eccentric (Ecc) resistance training. Male subjects engaged in unilateral resistance training utilizing isolated Ecc or Conc contractions. After 12 wk, muscle biopsies were analyzed for NG2+ and ALP+ pericytes, total Pax7+ SCs, activated SCs (Pax7+MyoD+), and differentiating myogenic cells (Pax7- MyoD+). NG2+ cells localized to CD31+ vessels and the majority coexpressed ALP. NG2+ pericyte quantity decreased following both Conc and Ecc training (P < 0.05). ALP+ pericyte quantity declined following Conc (P < 0.05) but not Ecc training. Conversely, total Pax7+ SC content was elevated following Conc only (P < 0.001), while Pax7+MyoD+ SC content was increased following Conc and Ecc (P < 0.001). Follow up analyses demonstrated that CD90+ and platelet-derived growth factor receptor-α (PDGFRα) + mononuclear cell proliferation was also increased in response to both Conc and Ecc training (P < 0.01). In summary, resistance training results in a decline in pericyte quantity and an increase in mesenchymal progenitor cell proliferation, and these events likely influence SC pool expansion and increased activation observed posttraining.

Original languageEnglish (US)
Pages (from-to)1053-1063
Number of pages11
JournalJournal of Applied Physiology
Volume119
Issue number10
DOIs
StatePublished - Nov 15 2015

Keywords

  • Eccentric exercise
  • Mesenchymal stem cells
  • Pericytes
  • Satellite cells

ASJC Scopus subject areas

  • Physiology
  • Physiology (medical)

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