Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater

Roland D. Cusick, Bill Bryan, Denny S. Parker, Matthew D. Merrill, Maha Mehanna, Patrick D. Kiely, Guangli Liu, Bruce E. Logan

Research output: Contribution to journalArticle

Abstract

A pilot-scale (1,000 L) continuous flow microbial electrolysis cell was constructed and tested for current generation and COD removal with winery wastewater. The reactor contained 144 electrode pairs in 24 modules. Enrichment of an exoelectrogenic biofilm required ∼60 days, which is longer than typically needed for laboratory reactors. Current generation was enhanced by ensuring adequate organic volatile fatty acid content (VFA/SCOD≥0.5) and by raising the wastewater temperature (31±1°C). Once enriched, SCOD removal (62±20%) was consistent at a hydraulic retention time of 1 day (applied voltage of 0.9 V). Current generation reached a maximum of 7.4 A/m 3 by the planned end of the test (after 100 days). Gas production reached a maximum of 0.19±0.04 L/L/day, although most of the product gas was converted to methane (86±6%). In order to increase hydrogen recovery in future tests, better methods will be needed to isolate hydrogen gas produced at the cathode. These results show that inoculation and enrichment procedures are critical to the initial success of larger-scale systems. Acetate amendments, warmer temperatures, and pH control during startup were found to be critical for proper enrichment of exoelectrogenic biofilms and improved reactor performance.

Original languageEnglish (US)
Pages (from-to)2053-2063
Number of pages11
JournalApplied Microbiology and Biotechnology
Volume89
Issue number6
DOIs
StatePublished - Mar 2011
Externally publishedYes

Fingerprint

Electrolysis
Waste Water
Gases
Biofilms
Hydrogen
Electrodes
Temperature
Volatile Fatty Acids
Methane
Acetates

Keywords

  • Bioelectricity
  • Bioenergy
  • Biohydrogen
  • Biomethane
  • Microbial electrolysis cell

ASJC Scopus subject areas

  • Biotechnology
  • Applied Microbiology and Biotechnology

Cite this

Cusick, R. D., Bryan, B., Parker, D. S., Merrill, M. D., Mehanna, M., Kiely, P. D., ... Logan, B. E. (2011). Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater. Applied Microbiology and Biotechnology, 89(6), 2053-2063. DOI: 10.1007/s00253-011-3130-9

Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater. / Cusick, Roland D.; Bryan, Bill; Parker, Denny S.; Merrill, Matthew D.; Mehanna, Maha; Kiely, Patrick D.; Liu, Guangli; Logan, Bruce E.

In: Applied Microbiology and Biotechnology, Vol. 89, No. 6, 03.2011, p. 2053-2063.

Research output: Contribution to journalArticle

Cusick, RD, Bryan, B, Parker, DS, Merrill, MD, Mehanna, M, Kiely, PD, Liu, G & Logan, BE 2011, 'Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater' Applied Microbiology and Biotechnology, vol 89, no. 6, pp. 2053-2063. DOI: 10.1007/s00253-011-3130-9
Cusick RD, Bryan B, Parker DS, Merrill MD, Mehanna M, Kiely PD et al. Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater. Applied Microbiology and Biotechnology. 2011 Mar;89(6):2053-2063. Available from, DOI: 10.1007/s00253-011-3130-9

Cusick, Roland D.; Bryan, Bill; Parker, Denny S.; Merrill, Matthew D.; Mehanna, Maha; Kiely, Patrick D.; Liu, Guangli; Logan, Bruce E. / Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater.

In: Applied Microbiology and Biotechnology, Vol. 89, No. 6, 03.2011, p. 2053-2063.

Research output: Contribution to journalArticle

@article{8c8ce90ecfe04c9c9c0eb67d4b70c3c1,
title = "Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater",
abstract = "A pilot-scale (1,000 L) continuous flow microbial electrolysis cell was constructed and tested for current generation and COD removal with winery wastewater. The reactor contained 144 electrode pairs in 24 modules. Enrichment of an exoelectrogenic biofilm required ∼60 days, which is longer than typically needed for laboratory reactors. Current generation was enhanced by ensuring adequate organic volatile fatty acid content (VFA/SCOD≥0.5) and by raising the wastewater temperature (31±1°C). Once enriched, SCOD removal (62±20%) was consistent at a hydraulic retention time of 1 day (applied voltage of 0.9 V). Current generation reached a maximum of 7.4 A/m 3 by the planned end of the test (after 100 days). Gas production reached a maximum of 0.19±0.04 L/L/day, although most of the product gas was converted to methane (86±6%). In order to increase hydrogen recovery in future tests, better methods will be needed to isolate hydrogen gas produced at the cathode. These results show that inoculation and enrichment procedures are critical to the initial success of larger-scale systems. Acetate amendments, warmer temperatures, and pH control during startup were found to be critical for proper enrichment of exoelectrogenic biofilms and improved reactor performance.",
keywords = "Bioelectricity, Bioenergy, Biohydrogen, Biomethane, Microbial electrolysis cell",
author = "Cusick, {Roland D.} and Bill Bryan and Parker, {Denny S.} and Merrill, {Matthew D.} and Maha Mehanna and Kiely, {Patrick D.} and Guangli Liu and Logan, {Bruce E.}",
year = "2011",
month = "3",
doi = "10.1007/s00253-011-3130-9",
volume = "89",
pages = "2053--2063",
journal = "Applied Microbiology and Biotechnology",
issn = "0175-7598",
publisher = "Springer Verlag",
number = "6",

}

TY - JOUR

T1 - Performance of a pilot-scale continuous flow microbial electrolysis cell fed winery wastewater

AU - Cusick,Roland D.

AU - Bryan,Bill

AU - Parker,Denny S.

AU - Merrill,Matthew D.

AU - Mehanna,Maha

AU - Kiely,Patrick D.

AU - Liu,Guangli

AU - Logan,Bruce E.

PY - 2011/3

Y1 - 2011/3

N2 - A pilot-scale (1,000 L) continuous flow microbial electrolysis cell was constructed and tested for current generation and COD removal with winery wastewater. The reactor contained 144 electrode pairs in 24 modules. Enrichment of an exoelectrogenic biofilm required ∼60 days, which is longer than typically needed for laboratory reactors. Current generation was enhanced by ensuring adequate organic volatile fatty acid content (VFA/SCOD≥0.5) and by raising the wastewater temperature (31±1°C). Once enriched, SCOD removal (62±20%) was consistent at a hydraulic retention time of 1 day (applied voltage of 0.9 V). Current generation reached a maximum of 7.4 A/m 3 by the planned end of the test (after 100 days). Gas production reached a maximum of 0.19±0.04 L/L/day, although most of the product gas was converted to methane (86±6%). In order to increase hydrogen recovery in future tests, better methods will be needed to isolate hydrogen gas produced at the cathode. These results show that inoculation and enrichment procedures are critical to the initial success of larger-scale systems. Acetate amendments, warmer temperatures, and pH control during startup were found to be critical for proper enrichment of exoelectrogenic biofilms and improved reactor performance.

AB - A pilot-scale (1,000 L) continuous flow microbial electrolysis cell was constructed and tested for current generation and COD removal with winery wastewater. The reactor contained 144 electrode pairs in 24 modules. Enrichment of an exoelectrogenic biofilm required ∼60 days, which is longer than typically needed for laboratory reactors. Current generation was enhanced by ensuring adequate organic volatile fatty acid content (VFA/SCOD≥0.5) and by raising the wastewater temperature (31±1°C). Once enriched, SCOD removal (62±20%) was consistent at a hydraulic retention time of 1 day (applied voltage of 0.9 V). Current generation reached a maximum of 7.4 A/m 3 by the planned end of the test (after 100 days). Gas production reached a maximum of 0.19±0.04 L/L/day, although most of the product gas was converted to methane (86±6%). In order to increase hydrogen recovery in future tests, better methods will be needed to isolate hydrogen gas produced at the cathode. These results show that inoculation and enrichment procedures are critical to the initial success of larger-scale systems. Acetate amendments, warmer temperatures, and pH control during startup were found to be critical for proper enrichment of exoelectrogenic biofilms and improved reactor performance.

KW - Bioelectricity

KW - Bioenergy

KW - Biohydrogen

KW - Biomethane

KW - Microbial electrolysis cell

UR - http://www.scopus.com/inward/record.url?scp=79952558400&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=79952558400&partnerID=8YFLogxK

U2 - 10.1007/s00253-011-3130-9

DO - 10.1007/s00253-011-3130-9

M3 - Article

VL - 89

SP - 2053

EP - 2063

JO - Applied Microbiology and Biotechnology

T2 - Applied Microbiology and Biotechnology

JF - Applied Microbiology and Biotechnology

SN - 0175-7598

IS - 6

ER -