TY - JOUR
T1 - Peptides in common bean fractions inhibit human colorectal cancer cells
AU - Luna Vital, Diego A.
AU - González De Mejía, Elvira
AU - Dia, Vermont P.
AU - Loarca-Piña, Guadalupe
N1 - Author Diego A. Luna Vital was supported by scholarship from the Consejo Nacional de Ciencia y Tecnologia CONACyT -Mexico. This research was supported by a Universidad Autónoma de Querétaro–University of Illinois Research Grant Program.
PY - 2014/8/15
Y1 - 2014/8/15
N2 - The aim of this study was to characterize peptides present in common bean non-digestible fractions (NDF) produced after enzymatic digestion and determine their antiproliferative action on human colorectal cancer cells. Five NDF peptides represented 70% of total protein (GLTSK, LSGNK, GEGSGA, MPACGSS and MTEEY) with antiproliferative activity on human colon cancer cells. Based on the antiproliferative effect, HCT116 cell line was most sensitive to bean Azufrado Higuera (IC50 = 0.53 mg/ml) and RKO to Bayo Madero (IC50 = 0.51 mg/ml) peptide extracts. Both cultivars increased significantly (p < 0.05) the expression of p53 in HCT116 by 76% and 68%, respectively. Azufrado Higuera modified the expression of cell cycle regulation proteins p21 and cyclin B1. Bayo Madero modified the expression of mitochondrial activated apoptotic proteins BAD, cytC, c-casp3, Survivin, BIRC7. Results suggest that peptides present in common bean NDF contributed to the antiproliferative effect on human colorectal cancer cells by modifying molecules involved in either cell cycle arrest or apoptosis.
AB - The aim of this study was to characterize peptides present in common bean non-digestible fractions (NDF) produced after enzymatic digestion and determine their antiproliferative action on human colorectal cancer cells. Five NDF peptides represented 70% of total protein (GLTSK, LSGNK, GEGSGA, MPACGSS and MTEEY) with antiproliferative activity on human colon cancer cells. Based on the antiproliferative effect, HCT116 cell line was most sensitive to bean Azufrado Higuera (IC50 = 0.53 mg/ml) and RKO to Bayo Madero (IC50 = 0.51 mg/ml) peptide extracts. Both cultivars increased significantly (p < 0.05) the expression of p53 in HCT116 by 76% and 68%, respectively. Azufrado Higuera modified the expression of cell cycle regulation proteins p21 and cyclin B1. Bayo Madero modified the expression of mitochondrial activated apoptotic proteins BAD, cytC, c-casp3, Survivin, BIRC7. Results suggest that peptides present in common bean NDF contributed to the antiproliferative effect on human colorectal cancer cells by modifying molecules involved in either cell cycle arrest or apoptosis.
KW - Bioactive peptides
KW - Colorectal cancer
KW - Common bean
KW - Non-digestible fraction
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U2 - 10.1016/j.foodchem.2014.02.050
DO - 10.1016/j.foodchem.2014.02.050
M3 - Article
C2 - 24679790
AN - SCOPUS:84896140078
SN - 0308-8146
VL - 157
SP - 347
EP - 355
JO - Food chemistry
JF - Food chemistry
ER -