Peak multiphoton excitation of mCherry using an optical parametric oscillator (OPO)

Tegy J. Vadakkan, James C. Culver, Liang Gao, Tiemo Anhut, Mary E. Dickinson

Research output: Contribution to journalArticlepeer-review

Abstract

mCherry is a red fluorescent protein which is bright, photostable, and has a low molecular weight. It is an attractive choice for multiphoton fluorescence imaging; however, the multiphoton excitation spectrum of mCherry is not known. In this paper we report the two photon excitation spectrum of mCherry measured up to 1190 nm in the near infrared (NIR) region. Skin tissues of transgenic mice that express mCherry were used in the experiments. mCherry in the tissues was excited with a Titanium:Sapphire laser and an optical parametric oscillator pumped by the Titanium:Sapphire laser. We found that the peak excitation of mCherry occurs at 1160 nm.

Original languageEnglish (US)
Pages (from-to)1103-1109
Number of pages7
JournalJournal of Fluorescence
Volume19
Issue number6
DOIs
StatePublished - Nov 2009

Keywords

  • Excitation peak
  • MCherry
  • Multiphoton
  • Optical parametric oscillator

ASJC Scopus subject areas

  • Biochemistry
  • Clinical Psychology
  • Social Sciences (miscellaneous)
  • Sociology and Political Science
  • Spectroscopy
  • Clinical Biochemistry
  • Law

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